Identification and Characterization of the Lipid Transport System in the Tarantula <i>Grammostola rosea</i>

dc.creatorLaino, Aldana
dc.creatorCunningham, Mónica Liliana
dc.creatorSuárez, Gustavo
dc.creatorGarcía, Carlos Fernando
dc.date2015
dc.date2020-09-16T16:28:05Z
dc.date.accessioned2023-07-14T21:57:18Z
dc.date.available2023-07-14T21:57:18Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/104770
dc.identifierhttp://hdl.handle.net/11336/49010
dc.identifierhttps://www.scirp.org/journal/paperinformation.aspx?paperid=52876
dc.identifierissn:2161-7597
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7445044
dc.descriptionLipids due to its hydrophobic nature are transported in the hemolymph mainly by lipoproteic fractions. In the present work we studied the lipoproteic fractions present in the hemolymph of the spider <i>Grammostola rosea</i>. Through ultracentrifugation two lipoproteic fractions are isolated, one of high density named Gr-HDL (δ = 1.13 - 1.15 g/ml) and the other of very high density called Gr-VHDL (δ = 1.24 - 1.27 g/ml) Gr-VHDL in hemolymph is majority in relation to Gr-HDL. In this sense Gr-VHDL fraction has 98.6% of hemolymphatic proteins, and 89.3% of lipids presents in the hemolymph. Both lipoproteic fractions possess phospholipids such as majority lipids (phosphatidylcholine and phosphatidylethanolamine) and 18:1, 16:0, 18:2 and 18:0 as the major fatty acids. In order to confirm the role played by lipoproteic fractions <i>in vitro</i> assays with different <sup>14</sup>C-lipid were performed. It was observed that Gr-VHDL takes up mainly free fatty acids and triacylglycerols unlike that observed for Gr-HDL in relation to phosphatidylcholine. Through electrophoresis it was observed that Gr-VHDL has three proteins: a predominant band of 68 kDa and two others of 99 and 121 kDa. Gr-HDL displayed a predominant band of 93 kDa, and other minority of 249 kDa. In conclusion, this study reports lipid characterization of the lipoproteic fractions present in the hemolymph of the tarantula, <i>G. rosea</i>. The role of each lipoproteic fraction in relation to lipid up- take is sustained by <i>in vitro</i> assays. Similarities and differences are found when it is compared to lipoproteins of only the three species of spiders studied.
dc.descriptionInstituto de Investigaciones Bioquímicas de La Plata
dc.formatapplication/pdf
dc.format9-20
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)
dc.subjectCiencias Exactas
dc.subjectQuímica
dc.subjectlipoproteins
dc.subjecthemocyanin
dc.subjectlipids
dc.subjectArachnids
dc.titleIdentification and Characterization of the Lipid Transport System in the Tarantula <i>Grammostola rosea</i>
dc.typeArticulo
dc.typeArticulo


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