| dc.creator | Morcelle del Valle, Susana Raquel | |
| dc.creator | Trejo, Sebastián Alejandro | |
| dc.creator | Canals, Francesc | |
| dc.creator | Avilés, Francesc X. | |
| dc.creator | Priolo de Lufrano, Nora Silvia | |
| dc.date | 2004-04 | |
| dc.date | 2020-06-08T18:06:04Z | |
| dc.date.accessioned | 2023-07-14T20:44:06Z | |
| dc.date.available | 2023-07-14T20:44:06Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/97753 | |
| dc.identifier | issn:572-3887 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7440391 | |
| dc.description | A cysteine endopeptidase, named funastrain c II, was isolated and characterized from the latex of Funastrum clausum (Asclepiadaceae). The molecular mass (mass spectrometry) of the protease was 23.636 kDa. The analysis of funastrain c II by SDS-PAGE revealed a single polypeptide chain. The enzyme showed a remarkable stability of its caseinolytic activity after incubation at temperatures as high as 70°C. Inhibition and activation assays indicated the cysteinic nature of the funastrain c II catalytic site. The optimum pH of funastrain c II enzymatic activity varied according to the substrate used (9.0–10.0 for casein and 6.2–6.8 for PFLNA). Kinetic parameters were determined for N-α-CBZ-Ala p-nitrophenyl ester (Km = 0.0243 mM, kcat = 1.5 s–1) and L-pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA; KM = 0.1011 mM, kcat = 0.9 s–1). The N-terminal sequence of funastrain c II showed considerable similarity to other proteases isolated from latex of different Asclepiadaceae species as well as to other cysteine proteinases belonging to the papain family. | |
| dc.description | Centro de Investigación de Proteínas Vegetales | |
| dc.format | application/pdf | |
| dc.format | 205-215 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.rights | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
| dc.subject | Biología | |
| dc.subject | Cysteine endopeptidases | |
| dc.subject | <i>Funastrum clausum</i> | |
| dc.subject | Latex | |
| dc.subject | Plant proteases | |
| dc.subject | Protein purification | |
| dc.title | Funastrain c II: A Cysteine Endopeptidase Purified from the Latex of <i>Funastrum clausum</i> | |
| dc.type | Articulo | |
| dc.type | Articulo | |
