Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation

dc.creatorLa Greca, Alejandro Damián
dc.creatorSolari, Claudia María
dc.creatorFurmento, Verónica Alejandra
dc.creatorLombardi, Antonella
dc.creatorBiani, María Celeste
dc.creatorAban, Cyntia Estefanía
dc.creatorMoro, Lucía Natalia
dc.creatorGarcía, Marcela Nilda
dc.creatorGuberman, Alejandra Sonia
dc.creatorSevlever, Gustavo
dc.creatorMiriuka, Santiago Gabriel
dc.creatorLuzzani, Carlos Daniel
dc.date2018-09
dc.date2020-08-05T18:14:25Z
dc.date.accessioned2023-07-14T20:33:37Z
dc.date.available2023-07-14T20:33:37Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/101459
dc.identifierhttps://ri.conicet.gov.ar/11336/93881
dc.identifierissn:1226-3613
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7439718
dc.descriptionMesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem cells (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free-based therapeutic approaches. Unlike cells, EVs do not elicit acute immune rejection, and they can be produced in large quantities and stored until ready to use. Although the therapeutic potential of MSC EVs has already been proven, a thorough characterization of MSC EVs is lacking. In this work, we used a label-free liquid chromatography tandem mass spectrometry proteomic approach to identify the most abundant proteins in EVs that are secreted from MSCs derived from PSCs (PD-MSCs) and from their parental induced PSCs (iPSCs). Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell–substrate adhesion. Moreover, compared to their respective cells, iPSCs and iPSC EVs share a greater proportion of proteins, while the PD-MSC proteome appears to be more specific. Correlation and principal component analysis consistently aggregate iPSCs and iPSC EVs but segregate PD-MSC and their EVs. Altogether, these findings suggest that during differentiation, compared with their parental iPSC EVs, PD-MSC EVs acquire a more specific set of proteins; arguably, this difference might confer their therapeutic properties.
dc.descriptionFacultad de Ciencias Médicas
dc.descriptionConsejo Nacional de Investigaciones Científicas y Técnicas
dc.formatapplication/pdf
dc.format1-12
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)
dc.subjectCiencias Exactas
dc.subjectExtracellular vesicles
dc.subjectMesenchymal stem cells
dc.subjectProteomic
dc.subjectDifferentiation
dc.titleExtracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
dc.typeArticulo
dc.typeArticulo


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