dc.creatorMedelnik, Jan Philip
dc.creatorRoensch, Kathleen
dc.creatorOkawa, Satoshi
dc.creatordel Sol, Antonio
dc.creatorChara, Osvaldo
dc.creatorMchedlishvili, Levan
dc.creatorTanaka, Elly M.
dc.date2018-06
dc.date2020-08-11T17:55:01Z
dc.date.accessioned2023-07-14T20:12:35Z
dc.date.available2023-07-14T20:12:35Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/101966
dc.identifierhttps://ri.conicet.gov.ar/11336/93820
dc.identifierissn:2213-6711
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7438330
dc.descriptionIn the developing nervous system, neural stem cells are polarized and maintain an apical domain facing a central lumen. The presence of apical membrane is thought to have a profound influence on maintaining the stem cell state. With the onset of neurogenesis, cells lose their polarization, and the concomitant loss of the apical domain coincides with a loss of the stem cell identity. Little is known about the molecular signals controlling apical membrane size. Here, we use two neuroepithelial cell systems, one derived from regenerating axolotl spinal cord and the other from human embryonic stem cells, to identify a molecular signaling pathway initiated by lysophosphatidic acid that controls apical membrane size and consequently controls and maintains epithelial organization and lumen size in neuroepithelial rosettes. This apical domain size increase occurs independently of effects on proliferation and involves a serum response factor-dependent transcriptional induction of junctional and apical membrane components. Medelnik and colleagues identify lysophosphatidic acid as a serum factor that has a major effect on the expansion of the apical domain of human ESC-derived neural progenitor cells, resulting in the formation of very large neural rosette-like structures that can be maintained for many passages in the absence of neuronal and glial differentiation if constantly supplied with LPA.
dc.descriptionInstituto de Física de Líquidos y Sistemas Biológicos
dc.formatapplication/pdf
dc.format1751-1765
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.subjectBiología
dc.subjectApical domain
dc.subjectCell polarization
dc.subjectHuman embryonic stem cells
dc.subjectLumen formation/organization
dc.subjectLysophosphatidic acid
dc.subjectNeural progenitor cells
dc.subjectNeural rosettes
dc.subjectNeural stem cells
dc.subjectNeuroepithelial cells
dc.titleSignaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells
dc.typeArticulo
dc.typeArticulo


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