Dietary fats significantly influence the survival of penumbral neurons in a permanent isquemic stroke rat model by modifying lipid mediators, inflammatory biomarkers, nos production, and redox-dependent apoptotic signals

dc.creatorLausada, Natalia Raquel
dc.creatorArnal, Nathalie
dc.creatorAstiz, Mariana
dc.creatorMarin, Maria Cristina
dc.creatorLofeudo, Juan Manuel
dc.creatorStringa, Pablo
dc.creatorTacconi de Alaniz, María Josefa
dc.creatorTacconi de Gómez Dumm, Irma Nelva
dc.creatorHurtado, Graciela Elisa
dc.creatorCristalli de Piñero, Norma
dc.creatorPallanza, Maria Cristina
dc.creatorIllara, Maria Eva
dc.creatorBozzarello, Enrique Gustavo
dc.creatorCristalli, Diana
dc.creatorMarra, Carlos Alberto
dc.date2015-11
dc.date2020-07-06T18:16:42Z
dc.date.accessioned2023-07-14T19:41:56Z
dc.date.available2023-07-14T19:41:56Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/100028
dc.identifierhttps://ri.conicet.gov.ar/11336/12434
dc.identifierissn:0899-9007
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7436333
dc.descriptionObjective: Brain stroke is the third most important cause of death in developed countries. We studied the effect of different dietary lipids on the outcome of a permanent ischemic stroke rat model. Methods: Wistar rats were fed diets containing 7% commercial oils (S, soybean; O, olive; C, coconut; G, grape seed) for 35 d. Stroke was induced by permanent middle cerebral artery occlusion. Coronal slices from ischemic brains and sham-operated animals were supravitally stained. Penumbra and core volumes were calculated by image digitalization after 24, 48, and 72 h poststroke. Homogenates and mitochondrial fractions were prepared from different zones and analyzed by redox status, inflammatory markers, ceramide, and arachidonate content, phospholipase A2, NOS, and proteases. Results: Soybean (S) and G diets were mainly prooxidative and proinflammatory by increasing the liberation of arachidonate and its transformation into prostaglandins. O was protective in terms of redox homeostatic balance, minor increases in lipid and protein damage, conservation of reduced glutathione, protective activation of NOS in penumbra, and net ratio of anti-to proinflammatory cytokines. Apoptosis (caspase-3, milli- and microcalpains) was less activated by O than by any other diet. Conclusion: Dietary lipids modulate NOS and PLA2 activities, ceramide production, and glutathione import into the mitochondrial matrix, finally determining the activation of the two main protease systems involved in programmed cell death. Olive oil appears to be a biological source for the isolation of protective agents that block the expansion of brain core at the expense of penumbral neurons.
dc.descriptionFacultad de Ciencias Médicas
dc.descriptionInstituto de Investigaciones Bioquímicas de La Plata
dc.formatapplication/pdf
dc.format1430-1442
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectBioquímica
dc.subjectDietary lipids
dc.subjectIschemic stroke
dc.subjectNeuronal death
dc.subjectLipid mediators
dc.subjectOxidative stress
dc.subjectInflammation
dc.titleDietary fats significantly influence the survival of penumbral neurons in a permanent isquemic stroke rat model by modifying lipid mediators, inflammatory biomarkers, nos production, and redox-dependent apoptotic signals
dc.typeArticulo
dc.typePreprint


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