| dc.creator | Ambrosis, Nicolás Martín | |
| dc.creator | Boyd, Chelsea D. | |
| dc.creator | O'Toole, George A. | |
| dc.creator | Fernández, Julieta | |
| dc.creator | Sisti, Federico | |
| dc.date | 2016 | |
| dc.date | 2019-12-02T17:01:49Z | |
| dc.date.accessioned | 2023-07-14T17:26:03Z | |
| dc.date.available | 2023-07-14T17:26:03Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/86497 | |
| dc.identifier | issn:1932-6203 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7427468 | |
| dc.description | Biofilm formation is important for infection by many pathogens. <i>Bordetella bronchiseptica</i> causes respiratory tract infections in mammals and forms biofilm structures in nasal epithelium of infected mice. We previously demonstrated that cyclic di-GMP is involved in biofilm formation in <i>B. bronchiseptica</i>. In the present work, based on their previously reported function in <i>Pseudomonas fluorescens</i>, we identified three genes in the <i>B. bronchiseptica</i> genome likely involved in c-di-GMP-dependent biofilm formation: brtA, lapD and lapG. Genetic analysis confirmed a role for BrtA, LapD and LapG in biofilm formation using microtiter plate assays, as well as scanning electron and fluorescent microscopy to analyze the phenotypes of mutants lacking these proteins. <i>In vitro</i> and <i>in vivo</i> studies showed that the protease LapG of <i>B. bronchiseptica</i> cleaves the N-terminal domain of BrtA, as well as the LapA protein of <i>P. fluorescens</i>, indicating functional conservation between these species. Furthermore, while BrtA and LapG appear to have little or no impact on colonization in a mouse model of infection, a <i>B. bronchiseptica</i> strain lacking the LapG protease has a significantly higher rate of inducing a severe disease outcome compared to the wild type. These findings support a role for c-di-GMP acting through BrtA/LapD/LapG to modulate biofilm formation, as well as impact pathogenesis, by <i>B. bronchiseptica</i>. | |
| dc.description | Facultad de Ciencias Exactas | |
| dc.description | Instituto de Biotecnologia y Biologia Molecular | |
| dc.format | application/pdf | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by/4.0/ | |
| dc.rights | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
| dc.subject | Ciencias Exactas | |
| dc.subject | Bordetella bronchiseptica | |
| dc.subject | respiratory tract infections | |
| dc.title | Homologs of the LapD-LapG c-di-GMP effector system control biofilm formation by <i>Bordetella bronchiseptica</i> | |
| dc.type | Articulo | |
| dc.type | Articulo | |
