| dc.creator | Bailon, H. | |
| dc.creator | León Janampa, N. | |
| dc.creator | Padilla, C. | |
| dc.creator | Hozbor, Daniela Flavia | |
| dc.date | 2016 | |
| dc.date | 2019-12-02T17:02:43Z | |
| dc.date.accessioned | 2023-07-14T17:25:32Z | |
| dc.date.available | 2023-07-14T17:25:32Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/86498 | |
| dc.identifier | issn:1471-2334 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7427437 | |
| dc.description | Background: As has occurred in many regions worldwide, in 2012 the incidence of pertussis increased in Perú. This epidemiologic situation has been associated with a waning vaccine-induced immunity and the adaptation of Bordetella pertussis to vaccine-induced immunity along with improved diagnostic methods. Methods: The study comprised a total of 840 pertussis-suspected cases reported in Perú during 2012. We summarize here the distribution of pertussis cases according to age and immunization status along with the immunization-coverage rate. Laboratory diagnosis was performed by culture test and real-time polymerase-chain reaction (PCR). B. pertussis bacteria recovered from infected patients were characterized by pulsed-field gel electrophoresis (PFGE), and the DNA sequencing of the pertussis-toxin (promoter and subunit A), pertactin, and fimbriae (fim2 and fim3) genes. Results: From the total pertussis-suspected cases, 191 (22.7 %) infections were confirmed by real-time PCR and 18 through cultivation of B. pertussis (2.1 %), while one infection of B. parapertussis (0.11 %) was also detected by culture. Pertussis was significantly higher in patients that had had 0-3 vaccine doses (pentavalent vaccine alone) than in those who had had 4-5 vaccine doses (pentavalent plus DwPT boosters) at 94.3 vs. 5.7 %, respectively (p < 0.00001). The relative risk (RR) for patients with 4-5 doses compared to those with fewer than 4 doses or no dose was 0.23 (95 % Confidence Interval: 0.11-0.44), while the vaccine effectiveness was 77 % and coverage 50.5 %. Genetic analysis of B. pertussis isolates from different Peruvian regions detected two clonal groups as identified by PFGE. Those two groups corresponded to the B. pertussis genotypes emerging worldwide ptxP3-ptxA1-prn2 or 9-fim3-1 and ptxP3-ptxA1-prn2 or 9-fim3-2. Conclusions: Two emerging B. pertussis genotypes similar to isolates involved in worldwide epidemics were detected in Perú. Low vaccine coverage (<50 %) and genetic divergence between the vaccine-producing strain and the local isolates could contribute to this pertussal epidemic. | |
| dc.description | Instituto de Biotecnologia y Biologia Molecular | |
| dc.format | application/pdf | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by/4.0/ | |
| dc.rights | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
| dc.subject | Ciencias Exactas | |
| dc.subject | Bordetella pertussis | |
| dc.subject | Genotype | |
| dc.subject | Pertussis | |
| dc.subject | Perú | |
| dc.subject | Pulsed-field gel electrophoresis | |
| dc.title | Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012 | |
| dc.type | Articulo | |
| dc.type | Articulo | |
