dc.contributor | Marcon Bruna H. | |
dc.contributor | Holetz Fabíola B. | |
dc.contributor | Eastman Guillermo | |
dc.contributor | Origa-Alves Ana Carolina | |
dc.contributor | Amorós Mariana Andrea | |
dc.contributor | Melo de Aguiar Alessandra | |
dc.contributor | Rebelatto Carmen K. | |
dc.contributor | Paulo R.S. Brofman | |
dc.contributor | Sotelo Silveira José Roberto, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. | |
dc.contributor | Dallagiovanna Bruno | |
dc.creator | Marcon, Bruna H. | |
dc.creator | Holetz, Fabíola B. | |
dc.creator | Eastman, Guillermo | |
dc.creator | Origa-Alves, Ana Carolina | |
dc.creator | Amorós, Mariana Andrea | |
dc.creator | Melo de Aguiar, Alessandra | |
dc.creator | Rebelatto, Carmen K. | |
dc.creator | Paulo R.S., Brofman | |
dc.creator | Sotelo Silveira, José Roberto | |
dc.creator | Dallagiovanna, Bruno | |
dc.date.accessioned | 2022-11-22T11:55:22Z | |
dc.date.accessioned | 2023-07-13T17:05:27Z | |
dc.date.available | 2022-11-22T11:55:22Z | |
dc.date.available | 2023-07-13T17:05:27Z | |
dc.date.created | 2022-11-22T11:55:22Z | |
dc.date.issued | 2017 | |
dc.identifier | Marcon, B, Holetz, F, Eastman, [y otros]. "Downregulation of the protein synthesismachinery is amajor regulatory event during early adipogenic differentiation of human adipose-derived stromal cells". Stem Cell Research. [en línea] 2017, 25:191-201.11 h. | |
dc.identifier | 1873-5061 | |
dc.identifier | https://hdl.handle.net/20.500.12008/34949 | |
dc.identifier | 10.1016/j.scr.2017.10.027 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7423497 | |
dc.description.abstract | Commitment of adult stemcells involves the activation of specific gene networks regulated fromtranscription to protein synthesis. Here, we used ribosome profiling to identify mRNAs regulated at the translational level, through both differential association to polysomes and modulation of their translational rates. We observed that translational regulation during the differentiation of human adipose-derived stromal cells (hASCs, also known as adipose-derived mesenchymal stem cells), a subset of which are stem cells, to adipocytes was a major regulatory event. hASCs showed a significant reduction ofwhole protein synthesis after adipogenic induction and a downregulation of the expression and translational efficiency of ribosomal proteins. Additionally, focal adhesion and cytoskeletal proteinswere downregulated at the translational level. This negative regulation of the
essential biological functions of hASCs resulted in a reduction in cell size and the potential of hASCs to migrate. We analyzed whether the inactivation of key translation initiation factors was involved in this observed major repression of translation. We showed that there was an increase in the hypo phosphorylated forms of 4E-BP1,
a negative regulator of translation, during early adipogenesis. Our results showed that extensive translational
regulation occurred during the early stage of the adipogenic differentiation of hASCs. | |
dc.language | en_US | |
dc.publisher | Elsevier B.V. | |
dc.relation | Stem Cell Research, 2017, 25:191-201. | |
dc.rights | Licencia Creative Commons Atribución (CC - By - NC - ND 4.0) | |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | |
dc.subject | hASCs | |
dc.subject | Adipogenesis | |
dc.subject | Ribosome profiling | |
dc.subject | Translation | |
dc.title | Downregulation of the protein synthesismachinery is amajor regulatory event during early adipogenic differentiation of human adipose-derived stromal cells | |
dc.type | Artículo | |