| dc.description.abstract | Physical exercise is an important health promotion strategy, especially due to the modulation it exerts on various organs and tissues. Exercise performance can be optimized by different strategies, especially food. So, identification of dietary strategies with a focus on improving the performance of physical exercise allowing the increasing its effects is a promising field of investigation. Pereskia aculeata Mill. (ora pro-nobis) is a perennial species of the Cactaceae family, used mainly as a vegetable unconventional hardwood. Although it has great productive and nutritional potential, in addition to medicinal properties is still underexplored and little known. The present study aims to objective to evaluate the effects of ora-pro-nobis flour on body composition, biochemical and molecular expression of genes associated with lipolysis/lipogenesis in tissue white adipose tissue and thermogenesis in brown adipose tissue of mice submitted to resistance training. The study was conducted with 14 mice were randomized into two groups: G1: control (CONTROL), fed with standard diet + resistance training; G2: (OPN), fed with a modified standard diet and added ora-pro nobis flour + resistance training.
The diet was produced from ora-pro-nobis flour. the flour was produced using leaves of the plant obtained in the region of Montes Claros, Minas Gerais, Brazil, which were washed, dried, dehydrated and crushed. The ora-pro-nobis flour diet presented final composition of 59.23% of carbohydrates, 28.07% of proteins and 12.7% of lipids, with an energy content of 3.03 Kcal/gram. The animals were submitted to 24 sessions of resistance training, composed of 6 sets (sets) of 8 repetitions with intervals of 120 seconds between sets. From the 13th section onwards, a weight was added to the training. attached to the animal's tail, corresponding to 50%
of its animal's body weight. In the study body, biochemical and histological parameters were measured, in addition to the expression of markers associated with thermogenesis in brown adipose tissue and adipogenesis/lipolysis in white adipose tissue by the qRT-PCR technique. The results showed that the animals supplemented with oro-pro-nobis flour had lower mean weight values (Control: 48.03±1.06 vs. OPN: 45.0±0.53), weight gain (Control: 6.11±1.23 vs. OPN:9.95±1.03) and visceral body adiposity (Control: 0.031±0.002 vs. OPN: 0.024±0.002); in addition, they had higher energy expenditure and lipid oxidation rate and had lower LDL-c levels. In the molecular evaluation, they showed a decrease in mRNA expression. for ACC and increase for PCG1α in white and brown adipose tissue, respectively. It is concluded that orapro-nobis flour can modulate the effects of physical exercise in mice, improving body,
biochemical and molecular parameters. | |
