| dc.creator | Derilus, Dieunel | |
| dc.creator | Massey, Steven (Consejero) | |
| dc.date | 2015-03-30T20:30:15Z | |
| dc.date | 2015-03-30T20:30:15Z | |
| dc.date | 2015-03-30T20:30:15Z | |
| dc.date.accessioned | 2017-03-17T16:54:42Z | |
| dc.date.available | 2017-03-17T16:54:42Z | |
| dc.identifier | http://hdl.handle.net/10586 /532 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/647652 | |
| dc.description | Due to its cost effectiveness and sustainability, anaerobic digestion (AD) has become a
widely adopted technology for wastewater treatment as well as bioenergy production. However,
knowledge of the structure and the dynamics of microbial communities involved in this process is
essential to improve system performance and optimize system operations. Here we investigated the
performance and the dynamics of the microbial communities in intermediate (10ppt) and high
(35ppt and 30ppt) salinity laboratory scale multi-stage anaerobic reactors fed with marine
macroalgae (Sargassum spp.) over 180 performance days by using barcoded 454 pyrosequencing
of 16S rRNA genes. The outcome of this survey may be summarized in five major findings: (1)
The intermediate salinity bioreactor is significantly more efficient in terms of biogas production
than the high salinity bioreactor; (2) The alpha diversity was found to decrease over time in
response to the adaptation of the microbial communities to different environmental factors; (3)
Fermentative Bacteroidetes and Firmicutes were the predominant and the most stable microbial
groups in the mature anaerobic reactors; (4) The
PCoA
reveals
significant
Beta
diversity
attributed
to
reactor
salinity
(R=
0.54,
p
=
0.002),
which is an indicator that salt concentration is
a strong ecological factor that shapes the biogas reactor microbial communities, (5) No
significant
partition
of
microbial
communities
was
observed
in
either
the
2L
two-‐stage
bioreactors,
or
the
15L
three-‐compartment
reactor
systems; and (6) Finally, in spite of the crucial role of
methanogenic Archaea for biogas production, they are present as a remarkably small fraction (1-
2%) of the mature bioreactor microbial communities. The characterization of the biogas produced
and additional meta-metabolomics studies are suggested in order to better understand the microbial
community functions in these systems. | |
| dc.language | en | |
| dc.subject | Bioreactors | |
| dc.subject | Sulfate Reducing Bacteria | |
| dc.subject | Next Generation Sequencing | |
| dc.subject | Microbial Communities | |
| dc.subject | Methanogenesis | |
| dc.subject | Biogas | |
| dc.subject | Wastewater treatment | |
| dc.subject | Environmental Sciences | |
| dc.title | Characterization of the Structure and Dynamics of Microbial Communities in Seawater Anaerobic Bioreactors by Using Next Generation Sequencing | |
| dc.type | Tesis | |
