| dc.creator | González Feliciano, José A. | |
| dc.creator | González Vargas, Carlos I. (Consejero) | |
| dc.date | 2014-06-24T19:54:18Z | |
| dc.date | 2014-06-24T19:54:18Z | |
| dc.date | 2014-06-24T19:54:18Z | |
| dc.date.accessioned | 2017-03-17T16:54:31Z | |
| dc.date.available | 2017-03-17T16:54:31Z | |
| dc.identifier | http://hdl.handle.net/10586 /475 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/647605 | |
| dc.description | An important aspect of the regulation of gene expression in eukaryotes is the post-transcriptional
control. Adenosine/Uridine-rich elements (AU-rich elements or AREs) within the 3'-untranslated
region (3'-UTR) of mRNAs play a crucial role in the post-transcriptional regulation by affecting
mRNA stability and/or translation. Cells involved in the immune response produce several
pleiotropic lymphokines that contain AU-rich elements in their mRNAs. Interlukin-3 is one of
these lymphokines that harbor an AU-rich element in its 3'-UTR. The aberrant expression of IL-3
is related to cancer, sickle cell disease and asthma, among others. At present, little is known
about the ARE-mediated post-transcriptional regulation of human IL-3. In order to fill this
scientific gap, the main focus of this dissertation is to understand the human IL-3 ARE-mediated
post-transcriptional regulatory pathway. This manuscript is divided in four chapters. Chapter I
consists of an introduction to the ARE-mediated post-transcriptional regulatory mechanism.
Chapter II focuses on the characterization of the IL-3 ARE post-transcriptional role. Chapter
III describes and characterizes the ARE-BPs that recognize the hIL-3 ARE and their roles in
mRNA stability and translation. Finally, Chapter IV elucidates the role of T-cell activation in
the hIL-3 ARE-mediated post-transcriptional regulation and its effects in the IL-3 ARE-binding
protein complexes. Altogether, the results presented in this dissertation provide new evidence
regarding the IL-3 ARE-mediated post-transcriptional regulation in human cells. | |
| dc.language | en | |
| dc.subject | Post-transcriptional Regulation | |
| dc.subject | mRNA Decay | |
| dc.subject | RNA regulatory elements | |
| dc.subject | RNA binding proteins | |
| dc.subject | Translational Control | |
| dc.subject | Cis-acting Elements | |
| dc.subject | Au-Rich elements | |
| dc.title | Identification and Characterization of Au-Rich Elements and Trans-Acting Factors Involved in the Posttranscriptional Regulation of Human Interleukin-3 | |
| dc.type | Tesis | |
