Pollen is produced by the male cone or flower organ (stamen), and represents the microgametophytic generation in spermatophytes. Pollen morphology has proved to be of significant taxonomicutility in different research areas,such as plant systematics, paleobotany, etnobotany, forensics, and aerobiology, among others. Today, there is no reference collection for pollen identification for Puerto Rico.This initiative aims to develop a pollen reference collection of the Island. At present, this collection supports two projects.