dc.creatorEspinoza, José
dc.creatorTimoteo, Olga
dc.creatorTimoteo, Olga
dc.creatorEspinoza, José
dc.date.accessioned2015-08-14T20:07:56Z
dc.date.accessioned2023-05-24T15:13:38Z
dc.date.available2015-08-14T20:07:56Z
dc.date.available2023-05-24T15:13:38Z
dc.date.created2015-08-14T20:07:56Z
dc.date.issued2005-08
dc.identifierTimoteo O, Espinoza J. Immune response in alpacas against Fasciola hepatica cysteine proteinases Fas1 and Fas2. Informe Científico Tecnológico. Volumen 4 (2004) p. 172-178.
dc.identifier1684-1662
dc.identifierhttps://hdl.handle.net/20.500.13054/444
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/6442687
dc.description.abstractA characterization of the humoral immune response of alpacas to Fasciola hepatica Fas1 and Fas2 antigens cysteine proteinases was performed over the course of 6 months of experimental infection. Six adult alpacas aged 1 to 2 years old received a single dose of 200 F. hepatica metacercariae, 2 non–infected alpacas were kept as control group. Peripheral eosinophilia in infected animals was greatly enhanced 6 weeks PI and later. A single peak of alanine aminotransferase (SGPT) was observed 4 week PI and aspartate aminotransferase (SGOT) elevated 3 weeks PI and later. All infected animals shed eggs 8 week PI and the number of flukes recovered at necropsy averaged 41 ± 4. Circulating IgG Abs against Fas1 and Fas2 were measured by ELISA. Fas2-ELISA detected the infection 10 days PI reaching to highest titer on 7-8 week PI and kept elevated until the end of infection. Fas1-ELISA detected the infection 2 weeks PI and followed the same pattern as Fas2-ELISA. Anti Fas2 IgG Abs were in higher titers and showed stronger avidity than anti Fas1 IgG Abs. In the present study we have established a F. hepatica experimental infection of alpacas that might be employed as a model to search for genes involved in the susceptibility to helminth infection in alpacas.
dc.languageeng
dc.publisherLima (Perú)
dc.publisherPE
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceInstituto Peruano de Energía Nuclear
dc.sourceRepositorio Institucional del Instituto Peruano de Energía Nuclear
dc.subjectAlpacas
dc.subjectFasciola hepatica
dc.subjectCamélidos sudamericanos
dc.subjectEnfermedades infecciosas
dc.titleImmune response in alpacas against Fasciola hepatica cysteine proteinases Fas1 and Fas2
dc.typeinfo:eu-repo/semantics/article


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