dc.creator | Espinoza, José | |
dc.creator | Timoteo, Olga | |
dc.creator | Timoteo, Olga | |
dc.creator | Espinoza, José | |
dc.date.accessioned | 2015-08-14T20:07:56Z | |
dc.date.accessioned | 2023-05-24T15:13:38Z | |
dc.date.available | 2015-08-14T20:07:56Z | |
dc.date.available | 2023-05-24T15:13:38Z | |
dc.date.created | 2015-08-14T20:07:56Z | |
dc.date.issued | 2005-08 | |
dc.identifier | Timoteo O, Espinoza J. Immune response in alpacas against Fasciola hepatica cysteine proteinases Fas1 and Fas2. Informe Científico Tecnológico. Volumen 4 (2004) p. 172-178. | |
dc.identifier | 1684-1662 | |
dc.identifier | https://hdl.handle.net/20.500.13054/444 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/6442687 | |
dc.description.abstract | A characterization of the humoral immune response of alpacas to Fasciola hepatica Fas1 and Fas2 antigens cysteine proteinases was performed over the course of 6 months of experimental infection. Six adult alpacas aged 1 to 2 years old received a single dose of 200 F. hepatica metacercariae, 2 non–infected alpacas were kept as control group. Peripheral eosinophilia in infected animals was greatly enhanced 6 weeks PI and later. A single peak of alanine aminotransferase (SGPT) was observed 4 week PI and aspartate aminotransferase (SGOT) elevated 3 weeks PI and later. All infected animals shed eggs 8 week PI and the number of flukes recovered at necropsy averaged 41 ± 4. Circulating IgG Abs against Fas1 and Fas2 were measured by ELISA. Fas2-ELISA detected the infection 10 days PI reaching to highest titer on 7-8 week PI and kept elevated until the end of infection. Fas1-ELISA detected the infection 2 weeks PI and followed the same pattern as Fas2-ELISA. Anti Fas2 IgG Abs were in higher titers and showed stronger avidity than anti Fas1 IgG Abs. In the present study we have established a F. hepatica experimental infection of alpacas that might be employed as a model to search for genes involved in the susceptibility to helminth infection in alpacas. | |
dc.language | eng | |
dc.publisher | Lima (Perú) | |
dc.publisher | PE | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Instituto Peruano de Energía Nuclear | |
dc.source | Repositorio Institucional del Instituto Peruano de Energía Nuclear | |
dc.subject | Alpacas | |
dc.subject | Fasciola hepatica | |
dc.subject | Camélidos sudamericanos | |
dc.subject | Enfermedades infecciosas | |
dc.title | Immune response in alpacas against Fasciola hepatica cysteine proteinases Fas1 and Fas2 | |
dc.type | info:eu-repo/semantics/article | |