Fluorescence in situ hybridization of rDNA, telomeric (TTAGGG)n and (GATA)n repeats in the red abalone Haliotis rufescens (Archaeogastropoda: Haliotidae)

dc.contributorJOSUE ALVAREZ BORREGO
dc.contributorMIGUEL ANGEL DEL RIO PORTILLA
dc.contributorIsmael Cross
dc.contributorAlvaro Merlo
dc.contributorLAUREANA REBORDINOS
dc.creatorCristian Gallardo
dc.date2005
dc.date.accessioned2023-03-16T14:29:29Z
dc.date.available2023-03-16T14:29:29Z
dc.identifierhttp://cicese.repositorioinstitucional.mx/jspui/handle/1007/1687
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/6229675
dc.description"The physical location of 18S-5.8S-28S rDNA, telomeric sequences with (TTAGGG)n DNA probe and (GATA)<sub>n</sub> microsatellites were performed by fluorescence in situ hybridization in chromosomes of red abalone Haliotis rufescens. The karyotype of red abalone showed a diploid number of 36 (8M+9SM+1ST). FISH performed with rDNA probe, showed the location of major ribosomal clusters in the terminal region of the large arms of two submetacentric pairs (chromosome 4 and 5). Localization of heteromorphisms of FISH-rDNA was found between chromosome homologues and sister chromatids in all metaphases analyzed. This indicates that rDNA clusters are variable within the red abalone genome. The variability in the NOR-bearing reported using silver staining in other gastropods and our result are discussed. In addition, the presence of microsatellite (TTAGGG)<sub>n</sub> and (GATA)n was demonstrated after FISH treatment by DNA probes. The telomeric sequence occurred at the ends of all mitotic chromosomes, while the (GATA)<sub>n</sub> repetitive was found on chromosomal interstitial zones as well as at the telomeres in abalone chromosomes."
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central
dc.relationinfo:eu-repo/semantics/altIdentifier/DOI/http://dx.doi.org/10.1111/j.1601-5223.2005.01909.x
dc.relationcitation:Gallardo Escárate,C.,Álvarez Borrego,J.,Del Río Portilla,M.A.,Cross,I.,Merlo,A.,Rebordinos,L.2005.Fluorescence in situ hybridization of rDNA, telomeric (TTAGGG)<sub>n</sub> and (GATA)<sub>n</sub> repeats in the red abalone Haliotis rufescens (Archaeogastropoda: Haliotidae).HereditasHereditas,142,73-79.doi:10.1111/j.1601-5223.2005.01909.x
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceHereditas, Vol. 142, Págs. 73-79
dc.subjectinfo:eu-repo/classification/WOS/Chromosomal Location, Cytogenetic Characterization, Mytilus-Galloprovincialis, Population-Genetics, Crassostrea-Gigas, Pacific Abalone, Donax-Trunculus, Ribosomal Dna, Black Abalone, California
dc.subjectinfo:eu-repo/classification/cti/1
dc.subjectinfo:eu-repo/classification/cti/22
dc.subjectinfo:eu-repo/classification/cti/2209
dc.subjectinfo:eu-repo/classification/cti/2209
dc.titleFluorescence in situ hybridization of rDNA, telomeric (TTAGGG)n and (GATA)n repeats in the red abalone Haliotis rufescens (Archaeogastropoda: Haliotidae)
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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