New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development

dc.creatorAcuña, Cintia Vanesa
dc.creatorRivas, Juan Gabriel
dc.creatorAguirre, Natalia Cristina
dc.creatorVillalba, Pamela Victoria
dc.creatorMartinez, Maria Carolina
dc.creatorGarcia, Martin Nahuel
dc.creatorHopp, Horacio Esteban
dc.creatorMarcucci Poltri, Susana Noemi
dc.date.accessioned2022-06-22T10:17:48Z
dc.date.accessioned2023-03-15T14:15:34Z
dc.date.available2022-06-22T10:17:48Z
dc.date.available2023-03-15T14:15:34Z
dc.date.created2022-06-22T10:17:48Z
dc.date.issued2020
dc.identifier2171-9845
dc.identifierhttps://doi.org/10.5424/fs/2020293-17074
dc.identifierhttp://hdl.handle.net/20.500.12123/12133
dc.identifierhttps://revistas.inia.es/index.php/fs/article/view/17074
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/6215087
dc.description.abstractAim of study: To validate and characterize new microsatellites or Simple Sequence Repeats (SSR) markers, located within genomic transcribed sequences related to growth and plant developmental traits, in Eucalyptus species. Area of study: Eucalyptus species from different Australian origins planted in Argentina. Materials and methods: In total, 134 SSR in 129 candidate genes (CG-SSR) involved in plant development were selected and physically mapped to the E. grandis reference genome by bioinformatic tools. Experimental validation and polymorphism analysis were performed on 48 individuals from E. grandis and interspecific hybrids (E. grandis x E. camaldulensis; E. grandis x E. tereticornis), E. globulus, E. maidenii, E. dunnii and E. benthamii. Main results: 131 out of 134 CG-SSR were mapped on the 11 chromosomes of E. grandis reference genome. Most of the 134 analyzed SSR (> 75%) were positively amplified and 39 were polymorphic in at least one species. A search of annotated genes within a 25 kbp up and downstream region of each SSR location retrieved 773 genes of interest. Research highlights: The new validated and characterized CG-SSR are potentially suitable for comparative QTL mapping, molecular marker-assisted breeding (MAB) and population genetic studies across different species within Symphyomyrtus subgenus.
dc.languageeng
dc.publisherInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
dc.relationinfo:eu-repograntAgreement/INTA/PNFOR-1104064/AR./Aplicación de herramientas moleculares para el uso y la conservación de la diversidad genética forestal.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceForest Systems 29 (3) : eSC08 (2020)
dc.subjectEucalyptus
dc.subjectMicrosatélites
dc.subjectCrecimiento
dc.subjectMicrosatellites
dc.subjectGrowth
dc.subjectGenetic Markers
dc.subjectMarcadores Genéticos
dc.titleNew validated eucalyptus SSR markers located in candidate genes involved in growth and plant development
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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