dc.creatorCarabajal Paladino, Leonale Suzel
dc.creatorPapeschi, Alba Graciela
dc.creatorLanzavecchia, Silvia Beatriz
dc.creatorCladera, Jorge Luis
dc.creatorBressa, María José
dc.date.accessioned2021-02-23T12:59:01Z
dc.date.accessioned2023-03-15T14:07:28Z
dc.date.available2021-02-23T12:59:01Z
dc.date.available2023-03-15T14:07:28Z
dc.date.created2021-02-23T12:59:01Z
dc.date.issued2013-07
dc.identifier1802-8829
dc.identifierhttps://doi.org/10.14411/eje.2013.054
dc.identifierhttp://hdl.handle.net/20.500.12123/8725
dc.identifierhttps://www.eje.cz/artkey/eje-201303-0003_Cytogenetic_characterization_of_Diachasmimorpha_longicaudata_Hymenoptera_Braconidae_a_parasitoid_wasp_used.php
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/6211755
dc.description.abstractDiachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). The entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. The high heterochromatin content differentiates D. longicaudata from other braconid species. Fluorescence in situ hybridization (FISH) using autologous 18S rDNA probes revealed six clusters of rDNA, i.e. six nucleolar organizer regions (NORs), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. This number is exceptionally high for Hymenoptera, which usually have two NORs in the diploid complement. It is noteworthy that these rDNA-FISH experiments represent the first use of this technique on a braconid species using autologous probes. Since Ag-NOR-bands were coincident with C-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. The physical characteristics of the D. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rDNA clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies.
dc.languageeng
dc.publisherCzech Academy of Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceEuropean Journal of Entomology 110 (3) : 401-409 (Julio 2013)
dc.subjectHymenoptera
dc.subjectBraconidae
dc.subjectBiosteres longicaudatus
dc.subjectCariotipos
dc.subjectHeterocromatina
dc.subjectADN ribosomal
dc.subjectCitogenética
dc.subjectParasitoides
dc.subjectAgentes de Control Biológico
dc.subjectKaryotypes
dc.subjectHeterochromatin
dc.subjectRibosomal DNA
dc.subjectCytogenetics
dc.subjectParasitoids
dc.subjectBiological Control Agents
dc.titleCytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución