dc.creatorAlmasia, Natalia Ines
dc.creatorBazzini, Ariel Alejandro
dc.creatorHopp, Horacio Esteban
dc.creatorVazquez Rovere, Cecilia
dc.date.accessioned2021-01-27T13:23:19Z
dc.date.accessioned2023-03-15T14:07:18Z
dc.date.available2021-01-27T13:23:19Z
dc.date.available2023-03-15T14:07:18Z
dc.date.created2021-01-27T13:23:19Z
dc.date.issued2008-05
dc.identifier1364-3703
dc.identifierhttps://doi.org/10.1111/j.1364-3703.2008.00469.x
dc.identifierhttp://hdl.handle.net/20.500.12123/8655
dc.identifierhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640289/
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/6211691
dc.description.abstractSnakin‐1 (SN1), a cysteine‐rich peptide with broad‐spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highly evolutionary conservation with 91–98% identity values. Potato plants (S. tuberosum subsp. tuberosum cv. Kennebec) were transformed via Agrobacterium tumefaciens with a construct encoding the S. chacoense SN1 gene under the regulation of the ubiquitous CaMV 35S promoter. Transgenic lines were molecularly characterized and challenged with either Rhizoctonia solani or Erwinia carotovora to analyse whether constitutive in vivo overexpression of the SN1 gene may lead to disease resistance. Only transgenic lines that accumulated high levels of SN1 mRNA exhibited significant symptom reductions of R. solani infection such as stem cankers and damping‐off. Furthermore, these overexpressing lines showed significantly higher survival rates throughout the fungal resistance bioassays. In addition, the same lines showed significant protection against E. carotovora measured as: a reduction of lesion areas (from 46.5 to 88.1% with respect to the wild‐type), number of fallen leaves and thickened or necrotic stems. Enhanced resistance to these two important potato pathogens suggests in vivo antifungal and antibacterial activity of SN1 and thus its possible biotechnological application.
dc.languageeng
dc.publisherWiley-Blackwell
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceMolecular plant pathology 9 (3) : 329-338. (Mayo 2008)
dc.subjectDisease Resistance
dc.subjectTransgenic Plants
dc.subjectPotatoes
dc.subjectResistencia a la Enfermedad
dc.subjectRhizoctonia Solani
dc.subjectPectobacterium Carotovorum
dc.subjectPlantas Transgénicas
dc.subjectPapa
dc.subjectSolanum
dc.titleOverexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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