dc.creatorFerre, Luis Bernardo
dc.creatorKjelland, M.E.
dc.creatorTaiyeb, A.M.
dc.creatorCampos‐Chillon, L.F.
dc.creatorRoss, Pablo J.
dc.date.accessioned2020-03-13T12:33:26Z
dc.date.accessioned2023-03-15T14:03:41Z
dc.date.available2020-03-13T12:33:26Z
dc.date.available2023-03-15T14:03:41Z
dc.date.created2020-03-13T12:33:26Z
dc.date.issued2020-03-07
dc.identifier0936-6768
dc.identifier1439-0531
dc.identifierhttps://doi.org/10.1111/rda.13667
dc.identifierhttp://hdl.handle.net/20.500.12123/6938
dc.identifierhttps://onlinelibrary.wiley.com/doi/abs/10.1111/rda.13667
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/6210033
dc.description.abstractCryopreservation of in vitro‐derived bovine embryos is a crucial step for the widespread reproduction and conservation of valuable high merit animals. Given the current popularity of bovine in vitro embryo production (IVP), there is a demand for a highly efficient ultra‐low temperature storage method in order to maximize donor ovum pick‐up (OPU) turn‐over, recipient availability/utilization and domestic/overseas commercial trading opportunities. However, IVP bovine embryos are still very sensitive to chilling and cryopreservation and despite recent progress, a convenient (simple and robust) protocol has not yet been developed. At the moment, there are two methods for bovine IVP embryo cryopreservation: slow programmable freezing and vitrification. Both of the aforementioned techniques have pros and cons. While controlled‐rate slow cooling can easily be adapted for direct transfer (DT), ice crystal formation remains an issue. On the other hand, vitrification solved this problem but the possibility of successful DT commercial incorporation remains to be determined. Moreover, simplification of the vitrification protocol (including warming) through the use of an in‐straw dilution without the use of a microscope is a prerequisite for its use under farm conditions. This review summarizes the bovine IVP embryo cryopreservation achievements, strengths and limitations of both freezing systems and prospective improvements to enhance cryosurvival, as well as perspectives on future directions of this assisted reproductive technology.
dc.languageeng
dc.publisherWiley
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.sourceReproduction in Domestic Animals (First published March 2020)
dc.subjectGanado Bovino
dc.subjectExperimentación in Vitro
dc.subjectCriopreservación
dc.subjectConservación de Embriones
dc.subjectCultivo de Embriones
dc.subjectCattle
dc.subjectIn Vitro Experimentation
dc.subjectCryopreservation
dc.subjectEmbryo Preservation
dc.subjectEmbryo Culture
dc.titleRecent progress in bovine In vitro‐derived embryo cryotolerance: impact of In vitro culture systems, advances in cryopreservation and future considerations
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/acceptedVersion


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