dc.creator | Ruiz, Vanesa | |
dc.creator | Baztarrica, Josefina | |
dc.creator | Rybicki, Edward P. | |
dc.creator | Meyers, Ann E. | |
dc.creator | Wigdorovitz, Andres | |
dc.date.accessioned | 2019-02-14T15:10:30Z | |
dc.date.accessioned | 2023-03-15T13:58:22Z | |
dc.date.available | 2019-02-14T15:10:30Z | |
dc.date.available | 2023-03-15T13:58:22Z | |
dc.date.created | 2019-02-14T15:10:30Z | |
dc.date.issued | 2018-12 | |
dc.identifier | 2215-017X | |
dc.identifier | https://doi.org/10.1016/j.btre.2018.e00283 | |
dc.identifier | http://hdl.handle.net/20.500.12123/4443 | |
dc.identifier | https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/6207644 | |
dc.description.abstract | Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.source | Biotechnology reports 20 : e00283. (December 2018) | |
dc.subject | Extractos de Hoja | |
dc.subject | Virus Fiebre Aftosa | |
dc.subject | Inmunogenética | |
dc.subject | Ratón | |
dc.subject | Leaf Extracts | |
dc.subject | Aphthovirus | |
dc.subject | immunogenetics | |
dc.subject | Mice | |
dc.title | Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |