MALDI-TOF MS identification of Prototheca algae associated with bovine mastitis

dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversity of Warsaw
dc.contributorUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:31:35Z
dc.date.accessioned2022-12-20T02:49:40Z
dc.date.available2022-04-29T08:31:35Z
dc.date.available2022-12-20T02:49:40Z
dc.date.created2022-04-29T08:31:35Z
dc.date.issued2021-01-01
dc.identifierJournal of Veterinary Diagnostic Investigation.
dc.identifier1943-4936
dc.identifier1040-6387
dc.identifierhttp://hdl.handle.net/11449/229296
dc.identifier10.1177/10406387211036459
dc.identifier2-s2.0-85112162300
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5409430
dc.description.abstractWe evaluated the use of MALDI-TOF MS for the identification of 3 major, dairy-associated Prototheca species, namely, Prototheca bovis (formerly P. zopfii genotype 2), P. blaschkeae, and P. ciferrii (formerly P. zopfii genotype 1). The MALDI-TOF MS spectra established for those species were introduced into the reference spectra library of the Bruker Biotyper MALDI-TOF MS analysis software. Next, 31 Prototheca isolates from Holstein cows with mastitis, from herds located in the midwestern area of São Paulo State, Brazil, were subjected to MALDI-TOF MS profiling. MALDI-TOF MS allowed identification of 22 of 27 P. bovis and 3 of 4 P. blaschkeae isolates with scores >2.0, with 5 of 27 P. bovis and 1 of 4 P. blaschkeae isolates identified only to the genus level. With our extended algae database, MALDI-TOF MS can contribute to quick and effective speciation of Prototheca from mastitis cases.
dc.languageeng
dc.relationJournal of Veterinary Diagnostic Investigation
dc.sourceScopus
dc.subjectBrazil
dc.subjectmass spectrometry
dc.subjectmicroalgae
dc.subjectPrototheca
dc.titleMALDI-TOF MS identification of Prototheca algae associated with bovine mastitis
dc.typeArtículos de revistas


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