dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T07:12:41Z
dc.date.accessioned2022-12-20T02:25:06Z
dc.date.available2022-04-29T07:12:41Z
dc.date.available2022-12-20T02:25:06Z
dc.date.created2022-04-29T07:12:41Z
dc.date.issued2013-11-27
dc.identifierLatin American Journal of Pharmacy, v. 32, n. 9, p. 1362-1369, 2013.
dc.identifier0326-2383
dc.identifierhttp://hdl.handle.net/11449/227317
dc.identifier2-s2.0-84888116995
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5407452
dc.description.abstractDibucaine (DBC), used mainly in topical formulations, is one of the most potent long-acting local anesthetics, but is also one of the more toxic. Recently, solid lipid nanoparticles and nanostructured lipid carriers have been attracting attention as promising drug delivery carriers. This study develops and validates an analytical HPLC method for quantifying the local anesthetic DBC associated to lipidic structures. Average sizes, polydispersion, surface charge and DBC encapsulation efficiency were analyzed. The DBC quantification was performed by using C18-reversed-phase column, a mobile phase with acetonitrile: triethylamine phosphate buffer and UV detection. The results show that the analytical methodology is accurate, reproducible and robust; the method was linear in the concentration range 1.5-30 μg/mL with a high correlation coefficient (r = 0.999).The nanoparticles presented mean diameters around 200 nm and high encapsulation efficiency for DBC (over 70% for SLN and NLC samples). This methodology can be useful for quantifying DBC in different nanostructured carriers.
dc.languageeng
dc.relationLatin American Journal of Pharmacy
dc.sourceScopus
dc.subjectDibucaine
dc.subjectHPLC
dc.subjectLipid carriers
dc.subjectMethod validation
dc.titleValidation of an HPLC method for the determination of dibucaine encapsulated in solid lipid nanoparticles and nanostructured lipid carriers
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución