Spontaneous canine transmissible venereal tumor: Association between different phenotypes and the insertion LINE-1/c-myc
Tumor venéreo transmisible canino espontáneo: Asociación entre diferentes fenotipos y la inserción LINE -1/c-myc
dc.contributor | Universidade Estadual Paulista (UNESP) | |
dc.date.accessioned | 2022-04-29T04:59:19Z | |
dc.date.accessioned | 2022-12-20T02:20:53Z | |
dc.date.available | 2022-04-29T04:59:19Z | |
dc.date.available | 2022-12-20T02:20:53Z | |
dc.date.created | 2022-04-29T04:59:19Z | |
dc.date.issued | 2012-01-01 | |
dc.identifier | Revista Colombiana de Ciencias Pecuarias, v. 25, n. 3, p. 402-408, 2012. | |
dc.identifier | 2256-2958 | |
dc.identifier | 0120-0690 | |
dc.identifier | http://hdl.handle.net/11449/226981 | |
dc.identifier | 2-s2.0-84867030788 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5407116 | |
dc.description.abstract | Objective: This study aimed to evaluate the LINE-1 transposon inserted in c-myc gene as a specific genetic alteration in cells of spontaneous canine Transmissible Venereal Tumor (TVT) with either lymphocytoid or plasmacytoid phenotypes. Methods: Tumoral biopsies from 35 dogs were collected by puncture or exfoliation. Polymerase Chain Reaction (PCR) was carried out with primers myc.s and LINE. A, specific to the LINE-1 segment to detect the presence of LINE-1/c-myc molecular marker. Results: Sequence alignment of DNA samples from lymphocytoid and plasmacytoid TVT cells did not show polymorphisms, and the comparison with sequences from the GenBank identified them as a LINE-1/c-myc rearrangement. Conclusions: Considering the aggressive nature of the plasmacytoid phenotype, there is no apparent relation between LINE-1/c-myc and the malignancy of TVT. Further studies are needed to establish molecular differences associated with the aggressiveness of the various phenotypes of canine TVT. | |
dc.language | eng | |
dc.language | spa | |
dc.relation | Revista Colombiana de Ciencias Pecuarias | |
dc.source | Scopus | |
dc.subject | Dogs | |
dc.subject | Oncogenes | |
dc.subject | Polymerase Chain Reaction | |
dc.subject | Transposition elements | |
dc.title | Spontaneous canine transmissible venereal tumor: Association between different phenotypes and the insertion LINE-1/c-myc | |
dc.title | Tumor venéreo transmisible canino espontáneo: Asociación entre diferentes fenotipos y la inserción LINE -1/c-myc | |
dc.type | Artículos de revistas |