Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.contributorAPTA/SAA
dc.date.accessioned2022-04-28T21:25:29Z
dc.date.accessioned2022-12-20T02:10:01Z
dc.date.available2022-04-28T21:25:29Z
dc.date.available2022-12-20T02:10:01Z
dc.date.created2022-04-28T21:25:29Z
dc.date.issued2010-01-01
dc.identifierJournal of Venomous Animals and Toxins Including Tropical Diseases, v. 16, n. 3, p. 414-420, 2010.
dc.identifier1678-9199
dc.identifierhttp://hdl.handle.net/11449/226102
dc.identifier10.1590/S1678-91992010000300007
dc.identifier2-s2.0-78049483980
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5406232
dc.description.abstractCanine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA and PCR. © CEVAP 2010.
dc.languageeng
dc.relationJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.sourceScopus
dc.subjectDiagnosis
dc.subjectDog
dc.subjectVisceral leishmaniasis
dc.titlePerformance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
dc.typeArtículos de revistas


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