First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype

dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorFaculdade de Medicina de São José do Rio Preto (FAMERP)
dc.contributorUNIRP
dc.contributorUnited States Department of Agriculture
dc.date.accessioned2020-12-12T02:24:13Z
dc.date.accessioned2022-12-19T21:12:20Z
dc.date.available2020-12-12T02:24:13Z
dc.date.available2022-12-19T21:12:20Z
dc.date.created2020-12-12T02:24:13Z
dc.date.issued2020-04-01
dc.identifierActa Tropica, v. 204.
dc.identifier1873-6254
dc.identifier0001-706X
dc.identifierhttp://hdl.handle.net/11449/201104
dc.identifier10.1016/j.actatropica.2020.105335
dc.identifier2-s2.0-85079234901
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5381738
dc.description.abstractThe protozoan parasite Toxoplasma gondii can infect virtually all warm-blooded animals worldwide but little is known of its infection in the endangered giant anteaters (Myrmecophaga tridactyla). The present study found antibodies to T. gondii in 13 of 23 free-living M. tridactyla from the northwest region of São Paulo state, Brazil, by the Modified Agglutination Test (MAT, cut-off titer 1:25). Unfrozen tissues of seven M. tridactyla were bioassayed in mice and viable T. gondii (strain designated TgMytrBrSP1) isolated from one seropositive giant anteater. To our knowledge, this is a new host record for T. gondii. Genotyping using PCR-RFLP revealed the Brazilian clonal Type BrIII genotype, and a unique non-archetypal genotype was revealed by microsatellite analysis.
dc.languageeng
dc.relationActa Tropica
dc.sourceScopus
dc.subjectGenotyping
dc.subjectGiant anteater
dc.subjectIsolation
dc.subjectMicrosatellites
dc.subjectPCR-RFLP
dc.subjectToxoplasmosis
dc.titleFirst isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype
dc.typeArtículos de revistas


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