dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.contributorBarretos Canc Hosp
dc.contributorMicroBiol Sect
dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorSao Francisco Univ
dc.date.accessioned2020-12-10T19:48:56Z
dc.date.accessioned2022-12-19T20:18:25Z
dc.date.available2020-12-10T19:48:56Z
dc.date.available2022-12-19T20:18:25Z
dc.date.created2020-12-10T19:48:56Z
dc.date.issued2020-02-01
dc.identifierAntonie Van Leeuwenhoek International Journal Of General And Molecular Microbiology. Dordrecht: Springer, v. 113, n. 2, p. 233-252, 2020.
dc.identifier0003-6072
dc.identifierhttp://hdl.handle.net/11449/196561
dc.identifier10.1007/s10482-019-01333-0
dc.identifierWOS:000512010300008
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5377198
dc.description.abstractOur aim was to identify less common non-fermenting gram-negative rods during the bioremediation process. Five genera were found: Advenella, Castellaniella, Kaistia, Pusillimonas and Sphingobacterium, for a total of 15 isolates. Therefore, we evaluated the applicability of four methods currently available for bacteria identification: (1) conventional biochemical methods, (2) the VITEK (R)-2 system, (3) MALDI-TOF mass spectrometry and (4) 16S rRNA gene sequencing. The biochemical methods and the VITEK (R)-2 system were reliable only for the Sphingobacterium isolate and solely at the genus level. Both MALDI-TOF mass spectrometry platforms (Bruker and VITEK (R) MS) did not achieve reliable identification results for any of these genera. 16S rRNA gene sequencing identified eight isolates to the species level but not to the subspecies level, when applicable. The remaining seven isolates were reliably identified through 16S rRNA gene sequencing to the genus level only. Our findings suggest that the detection and identification of less common genera (and species) that appeared at certain moments during the bioremediation process can be a challenge to microbiologists considering the most used techniques. In addition, more studies are required to confirm our results.
dc.languageeng
dc.publisherSpringer
dc.relationAntonie Van Leeuwenhoek International Journal Of General And Molecular Microbiology
dc.sourceWeb of Science
dc.subject16S rRNA
dc.subjectAdvenella
dc.subjectCastellaniella
dc.subjectKaistia
dc.subjectPusillimonas
dc.subjectSphingobacterium
dc.titleThe applicability of gene sequencing and MALDI-TOF to identify less common gram-negative rods (Advenella, Castellaniella, Kaistia, Pusillimonas and Sphingobacterium) from environmental isolates
dc.typeArtículos de revistas


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