dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | CIRIMAT Univ Toulouse | |
dc.contributor | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2020-12-10T17:26:58Z | |
dc.date.accessioned | 2022-12-19T20:01:47Z | |
dc.date.available | 2020-12-10T17:26:58Z | |
dc.date.available | 2022-12-19T20:01:47Z | |
dc.date.created | 2020-12-10T17:26:58Z | |
dc.date.issued | 2020-02-14 | |
dc.identifier | Nanotechnology. Bristol: Iop Publishing Ltd, v. 31, n. 8, 13 p., 2020. | |
dc.identifier | 0957-4484 | |
dc.identifier | http://hdl.handle.net/11449/195213 | |
dc.identifier | 10.1088/1361-6528/ab55c3 | |
dc.identifier | WOS:000514837000001 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5375850 | |
dc.description.abstract | Lanthanide (Ln) complexes emitting in the near-infrared (NIR) region have fostered great interest as upcoming optical tags owing to their high spatial and temporal resolution emission as well deeper light penetration in biological tissues for non-invasive monitoring. For use in live-cell imaging, lanthanide complexes with long-wavelength absorption and good brightness are especially critical. Light-harvesting ligands of Ln complexes are typically excited in the ultraviolet region, which in turn trigger simultaneously autofluorescence and long-exposition damage of living systems. The association of d-metalloligands rather than organic chromophores enables the excitation of NIR-emitting Ln complex occurs in the visible region. Taking advantage of the long-lived excited states and intense absorption band in the ultraviolet (UV) to NIR region of Ru(II), we successfully design a dual-emitting (in the visible and NIR region) d-f heterobinuclear complex based on Ru(II) metalloligand and Yb(III) complex. In addition, we developed luminescent nanohybrids by grafting of Ru(II)-Yb(III) heterobinuclear complexes containing silylated ligands on the surface of mesoporous and dense silica matrix. The nanomarkers were successfully applied for imaging of murine melanoma B16-F10 and neonatal human dermal fibroblast HDFn cell cultures by one-photon or two-photon absorption using laser scanning confocal microscopy. Great cellular uptake, low cytotoxicity and the possibility to achieve visible and NIR emission via two-photons excitation show that the nanohybrids are remarkable markers for in vitro and a potential tool for in vivo applications. | |
dc.language | eng | |
dc.publisher | Iop Publishing Ltd | |
dc.relation | Nanotechnology | |
dc.source | Web of Science | |
dc.subject | silylated d-f heterobinuclear complexes | |
dc.subject | grafting trialkoxysilyl group | |
dc.subject | mesoporous silica nanoparticles | |
dc.subject | visible and NIR luminescent nanohybrids | |
dc.subject | luminescent nanomarkers | |
dc.title | Luminescent nanohybrids based on silica and silylated Ru(II)-Yb(III) heterobinuclear complex: new tools for biological media analysis | |
dc.type | Artículos de revistas | |