Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniv Fed Maranhao
dc.contributorCtr Invest & Desarrollo
dc.date.accessioned2020-12-10T16:29:46Z
dc.date.accessioned2022-12-19T19:53:42Z
dc.date.available2020-12-10T16:29:46Z
dc.date.available2022-12-19T19:53:42Z
dc.date.created2020-12-10T16:29:46Z
dc.date.issued2000-07-01
dc.identifierQuimica Nova. Sao Paulo: Soc Brasileira Quimica, v. 23, n. 4, p. 466-471, 2000.
dc.identifier0100-4042
dc.identifierhttp://hdl.handle.net/11449/194551
dc.identifierWOS:000088609600006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5375187
dc.description.abstractELISAs have been applied to pesticide residue analysis due to their high sensitivity and selectivity. However, some ELISAs performance may be affected by matrix components. In this work, ELISA for carbaryl in water samples was checked for interference by naturally occurring fulvic acids. The results suggested that the high fulvic acid concentration (greater than or equal to 30 mg L-1) and acidic pH conditions (pH 4.0) interfere with the signal detection decreasing the method sensitivity. A dilution of the samples and adjust to pH 8.0 are appropriate to minimize the matrix interferences in the ELISA method. Good correlation between ELISA and HPLC-DAD results was observed.
dc.languagepor
dc.publisherSoc Brasileira Quimica
dc.relationQuimica Nova
dc.sourceWeb of Science
dc.subjectELISA
dc.subjectcarbaryl
dc.subjectfulvic acids
dc.titleDetermination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection
dc.typeArtículos de revistas


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