dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-10T19:09:04Z
dc.date.accessioned2022-12-19T19:10:01Z
dc.date.available2019-10-10T19:09:04Z
dc.date.available2022-12-19T19:10:01Z
dc.date.created2019-10-10T19:09:04Z
dc.date.issued2019-10-04
dc.identifier2045-2322
dc.identifierhttp://hdl.handle.net/11449/190700
dc.identifierhttps://doi.org/10.1038/s41598-019-50871-0
dc.identifier4741480538883395
dc.identifierhttps://orcid.org/0000-0002-2827-2038
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5371734
dc.description.abstractBiogenic synthesis of silver nanoparticles employing fungi offers advantages, including the formation of a capping from fungal biomolecules, which provides stability and can contribute to biological activity. In this work, silver nanoparticles were synthesized using Trichoderma harzianum cultivated with (AgNP-TS) and without enzymatic stimulation (AgNP-T) by the cell wall of Sclerotinia sclerotiorum. The nanoparticles were evaluated for the control of S. sclerotiorum. The specific activity of the T. harzianum hydrolytic enzymes were determined in the filtrates and nanoparticles. Cytotoxicity and genotoxicity were also evaluated. Both the nanoparticles exhibited inhibitory activity towards S. sclerotiorum, with no new sclerotia development, however AgNP-TS was more effective against mycelial growth. Both the filtrates and the nanoparticles showed specific enzymatic activity. Low levels of cytotoxicity and genotoxicity were observed. This study opens perspectives for further exploration of fungal biogenic nanoparticles, indicating their use for the control of S. sclerotiorum and other agricultural pests.
dc.languagepor
dc.publisherNature
dc.relationScientific Reports
dc.rightsAcesso aberto
dc.subjectSilver nanoparticles
dc.subjectAgricultural pests
dc.titleBiosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum
dc.typeArtículos de revistas


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