dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Federal University of Alfenas (UNIFAL) | |
dc.date.accessioned | 2019-10-06T16:30:34Z | |
dc.date.accessioned | 2022-12-19T18:51:04Z | |
dc.date.available | 2019-10-06T16:30:34Z | |
dc.date.available | 2022-12-19T18:51:04Z | |
dc.date.created | 2019-10-06T16:30:34Z | |
dc.date.issued | 2019-07-01 | |
dc.identifier | Archives of Oral Biology, v. 103, p. 19-25. | |
dc.identifier | 1879-1506 | |
dc.identifier | 0003-9969 | |
dc.identifier | http://hdl.handle.net/11449/189124 | |
dc.identifier | 10.1016/j.archoralbio.2019.05.006 | |
dc.identifier | 2-s2.0-85065707942 | |
dc.identifier | 5651874509493617 | |
dc.identifier | 0000-0002-2575-279X | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5370162 | |
dc.description.abstract | Objectives: To evaluate the effect of analogues of cationic peptides on the viability and the expression of phenotypic and genotypic markers of dentin mineralization in MDPC-23 odontoblast-like cells. Materials and methods: Cells were exposed to serial dilutions of analogues of cationic peptides hBD-3-1CV and KR-12-a5 compared to peptide LL-37 and their viability was assessed by methyltetrazolium assay. Next, peptides (0.78–62.5 μg/mL) were applied on the MDPC-23 cells for evaluating the total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition. Gene expression of mineralization markers (DSPP and DMP-1) was also determined by quantitative PCR. Results: LL-37 and hBD-3-1CV treatment did not affect cellular viability at concentrations below 62.5 μg/mL. KR-12-a5 reduced cell viability above 31.25 μg/mL. TP production was similar for all groups compared with the control group, except by hBD-3-1CV (at 15.62 μg/mL). LL-37 (at 62.5 μg/mL) induced higher ALP activity than control and other experimental groups. LL-37 and hBD-3-1CV, at 62.5 μg/mL and KR-12-a5 at 31.25 μg/mL stimulated the highest deposition of mineralized nodule. Overall, no statistical differences were observed between the groups for DSPP-1 and DMP-1 expressions. Conclusions: LL-37 was the only peptide that induced both ALP activity and mineralized nodules deposition, without affecting cell viability. None of peptides tested induced the expression of DSPP or DMP-1, genes commonly involved in active dentin mineralization. | |
dc.language | eng | |
dc.relation | Archives of Oral Biology | |
dc.rights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Cationic antimicrobial peptides | |
dc.subject | Cell culture | |
dc.subject | Cytotoxicity | |
dc.subject | Endodontics | |
dc.subject | Polymerase chain reaction | |
dc.title | Effect of analogues of cationic peptides on dentin mineralization markers in odontoblast-like cells | |
dc.type | Artículos de revistas | |