Use of mucus as a non-invasive sampling method for DNA barcoding of stingrays and skates (batoid elasmobranchs)

dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorNúcleo Integrado de Biotecnologia
dc.date.accessioned2019-10-06T16:21:22Z
dc.date.accessioned2022-12-19T18:47:55Z
dc.date.available2019-10-06T16:21:22Z
dc.date.available2022-12-19T18:47:55Z
dc.date.created2019-10-06T16:21:22Z
dc.date.issued2019-03-01
dc.identifierJournal of Fish Biology, v. 94, n. 3, p. 512-516, 2019.
dc.identifier1095-8649
dc.identifier0022-1112
dc.identifierhttp://hdl.handle.net/11449/188856
dc.identifier10.1111/jfb.13919
dc.identifier2-s2.0-85063145356
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5369894
dc.description.abstractIn this study we tested the use of mucus from five species of Neotropical marine batoid elasmobranchs to extract genomic DNA for barcoding and phylogenetic analysis. The DNA from all individuals sampled was successfully amplified and sequenced for molecular barcode, allowing 99–100% accuracy to the species level. This method proved to provide reliable and good-quality DNA for barcoding and phylogenetic analysis of Neotropical elasmobranchs, through rapid handling and with low disturbance to animals.
dc.languageeng
dc.relationJournal of Fish Biology
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectcytochrome c oxidase subunit 1
dc.subjectendangered elasmobranchs
dc.subjectmolecular markers
dc.subjectnon-lethal methods
dc.titleUse of mucus as a non-invasive sampling method for DNA barcoding of stingrays and skates (batoid elasmobranchs)
dc.typeArtículos de revistas


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