dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2019-10-06T16:11:03Z | |
dc.date.accessioned | 2022-12-19T18:44:01Z | |
dc.date.available | 2019-10-06T16:11:03Z | |
dc.date.available | 2022-12-19T18:44:01Z | |
dc.date.created | 2019-10-06T16:11:03Z | |
dc.date.issued | 2019-03-20 | |
dc.identifier | Gene, v. 689, p. 152-160. | |
dc.identifier | 1879-0038 | |
dc.identifier | 0378-1119 | |
dc.identifier | http://hdl.handle.net/11449/188526 | |
dc.identifier | 10.1016/j.gene.2018.12.012 | |
dc.identifier | 2-s2.0-85058836472 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5369564 | |
dc.description.abstract | Individuals carrying the ATC/TTC haplotype (Hap-1) in the interleukin 8 (IL8) gene were reported as more susceptible to chronic periodontitis (CP), an infectious disease associated with Gram-negative bacteria, in comparison to patients with the ATT/TTC haplotype (Hap-2). This study investigated the functionality of the IL8 haplotypes in lymphocytes and monocytes of individuals carrying the Hap-1 or Hap-2 IL8 haplotypes in the response to CP-associated Gram-negative bacteria (periodontopathogens). Peripheral blood was collected from 6 subjects carrying each haplotype, and their immune cells were challenged with periodontopathogens or phorbol 12-myristate 13-acetate (PMA) plus Ionomycin. Depending on the immune cell type (lymphocytes or monocyte-derived macrophages) the assessed outcomes were: phenotypical polarization, gene expression, phagocytic activity, chemotaxis and production of reactive oxygen species (ROS). Subjects carrying the Hap-1 haplotype showed increased expression of IL8 and TNFA and significantly skewing towards pro-inflammatory Th1/M1/Th17 phenotypes. There was increased percentage of ROS-producing monocyte-derived macrophages from individuals carrying the Hap-1 haplotype. Cells from individuals presenting the Hap-2 haplotype had an overall attenuated response to periodontopathogens, with a significant shift towards the Treg phenotype. In conclusion, the IL8 haplotypes showed to be functional both in monocyte-derived macrophages and lymphocytes. The Hap-1 haplotype previously associated with increased susceptibility to CP demonstrated greater skewing to pro-inflammatory Th1/M1/Th17 phenotypes and production of ROS. | |
dc.language | eng | |
dc.relation | Gene | |
dc.rights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Chemokines | |
dc.subject | Cytokines | |
dc.subject | Inflammation | |
dc.subject | Macrophage | |
dc.subject | Th1/Th2 cells | |
dc.title | Functionality of the Interleukin 8 haplotypes in lymphocytes and macrophages in response to gram-negative periodontopathogens | |
dc.type | Artículos de revistas | |