dc.contributor | Universidade de São Paulo (USP) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2019-10-06T16:07:30Z | |
dc.date.accessioned | 2022-12-19T18:42:46Z | |
dc.date.available | 2019-10-06T16:07:30Z | |
dc.date.available | 2022-12-19T18:42:46Z | |
dc.date.created | 2019-10-06T16:07:30Z | |
dc.date.issued | 2018-11-23 | |
dc.identifier | Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 24, n. 1, 2018. | |
dc.identifier | 1678-9199 | |
dc.identifier | 1678-9180 | |
dc.identifier | http://hdl.handle.net/11449/188420 | |
dc.identifier | 10.1186/s40409-018-0170-y | |
dc.identifier | S1678-91992018000100324 | |
dc.identifier | 2-s2.0-85057309005 | |
dc.identifier | S1678-91992018000100324.pdf | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5369458 | |
dc.description.abstract | Background: Snake venom phospholipases A2 (PLA2s) have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. This work aimed at the isolation and functional characterization of a PLA2 isolated from Bothrops jararaca venom, named BJ-PLA2-I. Methods and Results: For its purification, three consecutive chromatographic steps were used (Sephacryl S-200, Source 15Q and Mono Q 5/50 GL). BJ-PLA2-I showed acidic characteristics, with pI~ 4.4 and molecular mass of 14.2 kDa. Sequencing resulted in 60 amino acid residues that showed high similarity to other Bothrops PLA2s, including 100% identity with BJ-PLA2, an Asp49 PLA2 previously isolated from B. jararaca venom. Being an Asp49 PLA2, BJ-PLA2-I showed high catalytic activity, and also inhibitory effects on the ADP-induced platelet aggregation. Its inflammatory characterization showed that BJ-PLA2-I was able to promote leukocyte migration in mice at different concentrations (5, 10 and 20 μg/mL) and also at different response periods (2, 4 and 24 h), mainly by stimulating neutrophil infiltration. Furthermore, increased levels of total proteins, IL-6, IL-1β and PGE2 were observed in the inflammatory exudate induced by BJ-PLA2-I, while nitric oxide, TNF-α, IL-10 and LTB4 levels were not significantly altered. This toxin was also evaluated for its cytotoxic potential on normal (PBMC) and tumor cell lines (HL-60 and HepG2). Overall, BJ-PLA2-I (2.5-160 μg/mL) promoted low cytotoxicity, with cell viabilities mostly varying between 70 and 80% and significant values obtained for HL-60 and PBMC only at the highest concentrations of the toxin evaluated. Conclusions: BJ-PLA2-I was characterized as an acidic Asp49 PLA2 that induces acute local inflammation and low cytotoxicity. These results should contribute to elucidate the action mechanisms of snake venom PLA2s. | |
dc.language | eng | |
dc.relation | Journal of Venomous Animals and Toxins Including Tropical Diseases | |
dc.rights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Bothrops jararaca | |
dc.subject | Cytotoxicity | |
dc.subject | Inflammation | |
dc.subject | Phospholipase A2 | |
dc.subject | Snake venom | |
dc.title | Cytotoxic and inflammatory potential of a phospholipase A2 from Bothrops jararaca snake venom Suely V. Sampaio, Eliane C. Arantes, Marco A. Sartim | |
dc.type | Artículos de revistas | |