dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorQuality and Technology
dc.date.accessioned2019-10-06T15:30:12Z
dc.date.accessioned2022-12-19T18:28:42Z
dc.date.available2019-10-06T15:30:12Z
dc.date.available2022-12-19T18:28:42Z
dc.date.created2019-10-06T15:30:12Z
dc.date.issued2019-06-01
dc.identifierInternational Endodontic Journal, v. 52, n. 6, p. 829-837, 2019.
dc.identifier1365-2591
dc.identifier0143-2885
dc.identifierhttp://hdl.handle.net/11449/187247
dc.identifier10.1111/iej.13061
dc.identifier2-s2.0-85059638232
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5368285
dc.description.abstractAim: To investigate the biocompatibility, osteogenic bioactivity and mRNA expression of the osteo/odontogenic markers bone morphogenetic protein 2 (BMP-2), osteocalcin (OC) and alkaline phosphatase (ALP), induced by heparin in human dental pulp cells (hDPCs). Methodology: hDPCs were exposed to the heparin, and cell viability was assessed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT), and cell death was evaluated by flow cytometry. Osteogenic bioactivity was evaluated by the alkaline phosphatase (ALP) assay, and the detection of calcium deposits by alizarin red staining (ARS). The gene expression of BMP-2, OC and ALP was quantified with real-time PCR. Statistical analysis was performed with ANOVA and Bonferroni or Tukey post-test and t-test (α = 0.05). Results: Heparin had no cytotoxic effect and did not induce apoptosis. After 3 days, heparin had significantly higher ALP activity in comparison with the control (P < 0.05). Heparin had a significant (P < 0.05) stimulatory effect on the formation of mineralized nodules. BMP-2 and OC mRNA expressions were significantly higher in cells exposed to heparin than control group after 1 day (P < 0.05). Conclusions: Heparin was biocompatible in hDPCs, induced osteogenic bioactivity and enhanced mRNA expression of osteo/odontogenic markers BMP-2 and OC. These results suggest that heparin has potential to induce osteo/odontogenic cell differentiation of hDPCs.
dc.languageeng
dc.relationInternational Endodontic Journal
dc.rightsAcesso restrito
dc.sourceScopus
dc.subjectbone morphogenetic protein-2
dc.subjectcytotoxicity
dc.subjectdental pulp cells
dc.subjectheparin
dc.subjectosteogenic bioactivity
dc.titleHeparin is biocompatible and can induce differentiation of human dental pulp cells
dc.typeArtículos de revistas


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