Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis

dc.contributorUniversidade Estadual de Maringá (UEM)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-04T12:36:00Z
dc.date.accessioned2022-12-19T18:07:39Z
dc.date.available2019-10-04T12:36:00Z
dc.date.available2022-12-19T18:07:39Z
dc.date.created2019-10-04T12:36:00Z
dc.date.issued2019-02-01
dc.identifierFuture Microbiology. London: Future Medicine Ltd, v. 14, n. 3, p. 185-194, 2019.
dc.identifier1746-0913
dc.identifierhttp://hdl.handle.net/11449/185496
dc.identifier10.2217/fmb-2018-0277
dc.identifierWOS:000460352400004
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5366548
dc.description.abstractAim: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis. Materials & methods: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. Results: VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate. Conclusion: Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in M. tuberculosis.
dc.languageeng
dc.publisherFuture Medicine Ltd
dc.relationFuture Microbiology
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectefflux pump inhibitor
dc.subjectgene regulation
dc.subjectmultidrug transporters
dc.subjectMycobacterium tuberculosis
dc.subjectreal-time PCR
dc.subjectresistance
dc.subjectrifampicin
dc.subjectsynergism
dc.subjecttuberculosis
dc.subjectverapamil
dc.titleModulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis
dc.typeArtículos de revistas


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