A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells

dc.contributorUniv Fed Rural Pernambuco
dc.contributorFundacao Oswaldo Cruz
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade Federal de Pernambuco (UFPE)
dc.contributorUniv Fed Rio Janeiro
dc.date.accessioned2019-10-04T12:35:55Z
dc.date.accessioned2022-12-19T18:07:31Z
dc.date.available2019-10-04T12:35:55Z
dc.date.available2022-12-19T18:07:31Z
dc.date.created2019-10-04T12:35:55Z
dc.date.issued2018-12-17
dc.identifierNatural Product Research. Abingdon: Taylor & Francis Ltd, v. 32, n. 24, p. 2940-2944, 2018.
dc.identifier1478-6419
dc.identifierhttp://hdl.handle.net/11449/185484
dc.identifier10.1080/14786419.2017.1389932
dc.identifierWOS:000459884600012
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5366536
dc.description.abstractIn this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 nm. Amino acid sequences of peptides derived from in-gel digestion of MoFTI were determined. Both the extract and MoFTI caused lysis of T. cruzi trypomastigotes with LC50/24 h of 54.18 +/- 6.62 and 41.20 +/- 4.28 mu g/mL, respectively. High selectivity indices (7.9 to >12) for T. cruzi cells over murine peritoneal macrophages and Vero cells were found for the extract and MoFTI. The results show that MoFTI is a trypanocidal principle of the flower extract. [GRAPHICS] .
dc.languageeng
dc.publisherTaylor & Francis Ltd
dc.relationNatural Product Research
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.subjectMoringa oleifera
dc.subjectchagas disease
dc.subjectprotease inhibitor
dc.subjecttrypomastigote
dc.subjectcytotoxicity
dc.titleA trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells
dc.typeArtículos de revistas


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