The imprint as a non-invasive method of sampling for the molecular diagnosis of cutaneous leishmaniasis in Colombia's military population

La impronta como método no invasivo de toma de muestra para el diagnóstico molecular de la leishmaniosis cutánea en población militar de Colombia;
In print como método não invasivo de coleta de amostra para o diagnóstico molecular da leishmaniose cutânea em população militar da Colômbia

dc.creatorMéndez Bejarano, Claudia Patricia
dc.creatorPérez Rico, Julie Janeth
dc.creatorRodriguez Angarita, Omaira
dc.creatorVelandia Rodríguez, Daniel Augusto
dc.creatorRomero Barbosa, Yanira Andrea
dc.creatorAlvarado Flechas, María Teresa
dc.creatorValbuena Pinzón, Tania Mabel
dc.date2019-12-23
dc.date.accessioned2022-12-15T18:28:27Z
dc.date.available2022-12-15T18:28:27Z
dc.identifierhttps://revistas.unimilitar.edu.co/index.php/rmed/article/view/3037
dc.identifier10.18359/rmed.3037
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5357437
dc.descriptionTaking into account that the traditional methods of sampling and diagnosis for cutaneous leishmaniasis , have limitations, such as direct smear, whose sensitivity depends on the professional's expertise, the lesion aspiration that can be used to detect parasites in the lamina, dna or cultures takes a long time and is demanding, and the biopsy of the lesion that is invasive and painful were compared with the imprinting method on the filter paper of the ulcerative lesion against the traditional method of aspiration by means of the conventional pcr technique using as a target a dna region of the parasite kinetoplast. In this present work, pcr obtained an imprinting sensitivity of 90.07% compared to the aspirate of 86.3%, which, besides being a non-invasive sampling method, with few transport requirements, it can be taken directly in the area of operations at a very low cost, which turns out to be beneficial to be used in patients with cutaneous leishmaniasis of the National Army of Colombia, who are in different operations areas.en-US
dc.descriptionTeniendo en cuenta que los métodos tradicionales de toma de muestra y diagnóstico para la leishmaniosis cutánea presentan limitaciones, como el frotis directo, cuya sensibilidad depende de la pericia del profesional, el aspirado de lesión que puede ser usado para detección de parásitos en lámina, su adn o para cultivos es demorado y exigente, y la biopsia de la lesión que es invasiva y dolorosa se comparó con el método de impronta en papel filtro de la lesión ulcerativa contra el método tradicional de aspirado mediante la técnica de pcr convencional utilizando como blanco una región del adn del kinetoplasto del parásito. En el presente trabajo, la pcr obtuvo una sensibilidad para impronta del 90,07% comparado con el aspirado, el 86,3%, que, además, por ser un método de toma de muestra no invasivo, con pocas exigencias para el transporte, se puede tomar directamente en el área de operaciones a muy bajo costo, resulta ser beneficioso para ser usado en los pacientes con leishmaniosis cutánea del Ejército Nacional de Colombia, que se encuentran en las diferentes áreas de operaciones.es-ES
dc.descriptionConsiderando que os métodos tradicionais de coleta de amostra e diagnóstico para a leishmaniose cutânea apresentam limitações, como exame direto de esfregaços, cuja sensibilidade depende da perícia do profissional, o raspado de lesão que pode ser usado para a detecção de parasitas em lâmina, seu dna ou para culturas é demorado e exigente, e a biopsia da lesão que é invasiva e dolorosa, comparou-se com o método in print em papel filtro da lesão ulcerativa contra o método tradicional de aspirado mediante a técnica de pcr convencional utilizando como alvo uma região do dna do cinetoplasto do parasita. No presente trabalho, a pcr obteve uma sensibilidade para in print de 90,07% comparado com o aspirado, 86,3%, que, além disso, por ser um método de coleta de amostra não invasivo, com poucas exigências para o transporte, pode ser coletado diretamente na área de operações a muito baixo custo, resulta ser benéfico para ser usado nos pacientes com leishmaniose cutânea do Exército Nacional da Colômbia, que se encontram nas diferentes áreas de operações.pt-BR
dc.formattext/xml
dc.formatapplication/pdf
dc.languagespa
dc.publisherUniversidad Militar Nueva Granadaes-ES
dc.relationhttps://revistas.unimilitar.edu.co/index.php/rmed/article/view/3037/3902
dc.relationhttps://revistas.unimilitar.edu.co/index.php/rmed/article/view/3037/4089
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dc.rightsDerechos de autor 2019 Revista Medes-ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0es-ES
dc.sourceRevista Med; Vol. 26 No. 2 (2018): july - december; 15-21en-US
dc.sourceRevista Med; Vol. 26 Núm. 2 (2018): julio - diciembre; 15-21es-ES
dc.source1909-7700
dc.source0121-5256
dc.titleThe imprint as a non-invasive method of sampling for the molecular diagnosis of cutaneous leishmaniasis in Colombia's military populationen-US
dc.titleLa impronta como método no invasivo de toma de muestra para el diagnóstico molecular de la leishmaniosis cutánea en población militar de Colombiaes-ES
dc.titleIn print como método não invasivo de coleta de amostra para o diagnóstico molecular da leishmaniose cutânea em população militar da Colômbiapt-BR
dc.typeinfo:eu-repo/semantics/article
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