In vitro production of lilium (Lilium sp) microbulblets

Producción in vitro de microbulbos de lirio (Lilium sp)

dc.creatorCastro, Dagoberto
dc.creatorLondoño, Santiago
dc.date2008-01-01
dc.date.accessioned2022-12-15T14:44:37Z
dc.date.available2022-12-15T14:44:37Z
dc.identifierhttps://revistas.unicordoba.edu.co/index.php/temasagrarios/article/view/659
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5353750
dc.descriptionAn efficient method was developed for the propagation of Lilium sp by means of bulb scales as explants of the hybrid “Casablanca”. The sections of scales were disinfected during 15 minutes with sodium hypoclorite (0.5%) and peroxide of hydrogen (0.03%) during two minutes. The explants were placed on the medium consisting of Murashige and Skoog salts supplemented with BAP (0.05 mg L-1). For the induction and diferenciatión of microbulblets, it was found that NAA (0.1 mg L-1) showed the best effect. In the proliferation phase, kinetina (1.0 mg L-1) plus sucrose (60 g L-1) induced the highest bulblet number (3.9) with 75% differentiated explantes and the highest fresh and dry masses values as well. During the acclimatization phase 96.4% surviving was achieved and 45 days after establishment formed the first minibulbleten-US
dc.descriptionSe desarrolló un método eficiente para la propagación de Lilium sp mediante el empleo de escamas tomadas a partir de bulbos del híbrido “Casablanca”. Las secciones de escamas se desinfectaron con hipoclorito de sodio (0.5%) durante 15 minutos y peróxido de hidrógeno (0.03%) durante dos minutos y se establecieron en un medio de cultivo compuesto por las sales minerales de Murashige y Skoog y suplementado conbenciladenina (0.05 mg L-1). Para la inducción y diferenciación de microbulbos se encontró que el ácido naftalenacético (0.1 mg L 1), presentó el mejor efecto sobre el porcentaje de explantes diferenciados y el número de microbulbos formados por explante. En la fase de proliferación cuando se empleó la kinetina (1.0 mg L-1) más sacarosa (60 g L-1) presentó el mayor número de bulbos (3.9), con un 75% de explantes diferenciados y los mayores valores de masas seca y fresca. Durante la fase de aclimatización se obtuvo un 96.4% de prendimiento y al cabo de 45 días se formó el primer minibulbo.es-ES
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languagespa
dc.languageeng
dc.publisherUniversidad de Córdobaes-ES
dc.relationhttps://revistas.unicordoba.edu.co/index.php/temasagrarios/article/view/659/775
dc.relationhttps://revistas.unicordoba.edu.co/index.php/temasagrarios/article/view/659/4295
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dc.rightsDerechos de autor 2016 Temas Agrarioses-ES
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0es-ES
dc.sourcesour topics; Vol. 13 No. 1 (2008): Revista Temas Agrarios Volumen 13(1) (Enero - Junio 2008); 5-13en-US
dc.sourceTemas Agrarios; Vol. 13 Núm. 1 (2008): Revista Temas Agrarios Volumen 13(1) (Enero - Junio 2008); 5-13es-ES
dc.source2389-9182
dc.subjectliliumen-US
dc.subjectdirect organogenesisen-US
dc.subjectregenerationen-US
dc.subjectmicrobulbleten-US
dc.subjectminibulblet.en-US
dc.subjectLiliumes-ES
dc.subjectorganogénesis directaes-ES
dc.subjectregeneraciónes-ES
dc.subjectminibulboses-ES
dc.subjectmicrobulboses-ES
dc.titleIn vitro production of lilium (Lilium sp) microbulbletsen-US
dc.titleProducción in vitro de microbulbos de lirio (Lilium sp)es-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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