Genetic variation in two candidate genes against gastrointestinal parasites in Colombian Hair Sheep

Variación genética en dos genes candidatos contra parásitos gastrointestinales en Ovinos de Pelo Colombiano

dc.creatorHernández-Herrera, Darwin
dc.creatorLenis-Valencia, Claudia
dc.creatorMontes-Vergara, Donicer
dc.date2022-07-15
dc.date.accessioned2022-12-15T14:43:55Z
dc.date.available2022-12-15T14:43:55Z
dc.identifierhttps://revistamvz.unicordoba.edu.co/article/view/2747
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5353529
dc.descriptionObjective. To characterize two SNP-type genetic polymorphisms in the GLI1 (rs411868094) and IL20RA (rs419463995) genes, candidates for resistance against gastrointestinal parasites in two biotypes of Colombian hair sheep. Materials and methods. From the DNA bank of the Animal Genetics Laboratory of the University of Sucre, 167 samples, belonging to the Ethiopian (n=94) and Sudan (n=73) biotypes, were analyzed by PCR and subsequent bidirectional sequencing of two SNPs in the GLI1 (T>G) and IL20RA (G>A) genes. Allelic and genotypic frequencies, observed (Ho) and expected (He) heterozygosity, F index, and Hardy-Weinberg equilibrium deviations (EHW) were calculated using the GENALEX version 6.5 program. Results. For the GLI1 locus, the mean genotypic frequencies were 0.155±0.07, 0.370±0.07, and 0.475±0.07 for GG, GT, and TT, respectively. In the Ethiopian biotype, the highest frequencies of the genotype of interest (GG) were found. For the IL20RA locus, the AA and AG genotypes had similar and the highest frequency (0.465±0.03) compared to the GG genotype (0.110±0.01). The genotype of interest at this locus (AA) was the most frequent in both OPC biotypes. Conclusions. The alleles of interest associated with low FEC had a low frequency for the GLI1 gene, but a high frequency for IL20RA. The Ethiopian OPC biotype showed the highest frequencies of the genotypes of interest.en-US
dc.descriptionObjetivo. Caracterizar dos polimorfismos genéticos tipo SNP en los genes GLI1 (rs411868094) y IL20RA (rs419463995) candidatos a la resistencia contra parásitos gastrointestinales en dos biotipos de ovinos de pelo colombiano. Materiales y métodos. Del banco de ADN del laboratorio de Genética Animal de la Universidad de Sucre, se analizaron 167 muestras de ovino de pelo colombiano (OPC), pertenecientes a los biotipos Etíope (n=94) y Sudán (n=73), mediante PCR y secuenciamiento bidireccional dos SNPs en los genes GLI1 (T>G) y IL20RA (G>A). Se calcularon las frecuencias alélicas y genotípicas, la heterocigocidad observada (Ho) y esperada (He), el índice F y las desviaciones del equilibrio de Hardy-Weinberg (EHW) con el programa GENALEX versión 6.5. Resultados. Para el gen GLI1 para todo el OPC las frecuencias genotípicas promedio fueron 0.155±0.07, 0.370±0.07 y 0.475±0.07 para GG, GT y TT, respectivamente. En el biotipo Etíope, se encontraron las frecuencias más altas del genotipo GG. Para el gen IL20RA en todo el OPC, los genotipos AA y AG tuvieron similar frecuencia (0.465±0.03) y el genotipo GG mostró la frecuencia más baja (0.110±0.01). Conclusiones. Las variantes genéticas analizadas fueron polimórficas. Según los reportes de literatura, los alelos de interés por su mejor desempeño contra los parásitos gastrointestinales, tuvieron baja frecuencia en el gen GLI1, pero alta frecuencia en el IL20RA.es-ES
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dc.languagespa
dc.languageeng
dc.publisherUniversidad de Córdobaes-ES
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dc.rightsDerechos de autor 2022 Darwin Hernández-Herrera, Claudia Lenis-Valencia, Donicer Montes-Vergaraes-ES
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0es-ES
dc.sourceJournal MVZ Cordoba; Vol. 27 No. s (2022): Vol. 27 Supl (2022): Revista MVZ Córdoba Volumen 27(Supl) 2022; e2747en-US
dc.sourceRevista MVZ Córdoba; Vol. 27 Núm. s (2022): Vol. 27 Supl (2022): Revista MVZ Córdoba Volumen 27(Supl) 2022; e2747es-ES
dc.source1909-0544
dc.source0122-0268
dc.source10.21897/rmvz.v27.ns.2022
dc.subjectGenetic diversityen-US
dc.subjectgenetic resistanceen-US
dc.subjectanimal genetic resourcesen-US
dc.subjectDiversidad genéticaes-ES
dc.subjectresistencia genéticaes-ES
dc.subjectrecursos genéticos animaleses-ES
dc.titleGenetic variation in two candidate genes against gastrointestinal parasites in Colombian Hair Sheepen-US
dc.titleVariación genética en dos genes candidatos contra parásitos gastrointestinales en Ovinos de Pelo Colombianoes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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