Immunohistochemical localization of transient receptor potential vanilloid-type 5 in ameloblasts of Swiss mice

Localización inmunohistoquímica de receptores de potencial transitorio tipo vaniloide 5 en ameloblastos de ratones Swiss

dc.creatorSimancas-Escorcia, Victor Hugo
dc.creatorDíaz-Caballero, Antonio
dc.creatorMartinez-Martinez, Adel
dc.date2022-09-01
dc.date.accessioned2022-12-15T14:43:53Z
dc.date.available2022-12-15T14:43:53Z
dc.identifierhttps://revistamvz.unicordoba.edu.co/article/view/2681
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5353520
dc.descriptionObjective. To determine the localization of transient receptor potential vanilloid 5 (TRPV5) in the ameloblasts of Swiss mice. Materials and methods. In vitro experimental study where paraffin sections of 12 continuously growing incisors of 7-day-old male Swiss mice were analyzed. Developmental stages of dental enamel formation were identified by hematoxylin-eosin staining. Immunodetection of the transient receptor potential vanilloid 5 was performed using the primary polyclonal antibody anti-TRPV5. Observations were carried out using a Leica DM 500 microscope. Results. The different stages involved in the formation of dental enamel were identified, including the secretory and maturation stages. In them, it was evidenced that the ameloblasts were elongated cells with oval nuclei in a proximal position and with developed areas of medial and distal secretion. TRPV5 immunolabeling was visualized in the cell membrane and cytoplasm of the secretory and maturation ameloblasts of all mice tested. TRPV5s were also immunolocalized in odontoblasts, vascular endothelium, and pulp cells. Conclusions. The transient receptor potential vanilloid 5 is located on the secretory and maturing ameloblasts of Swiss mice. In particular, TRPV5s are immunodetected in odontoblasts, vascular endothelium, and dental pulp cells.en-US
dc.descriptionObjetivo. Determinar la localización de los receptores de potencial transitorio de tipo vaniloide-5 (TRPV5) en los ameloblastos de ratones Swiss. Materiales y métodos. Estudio experimental In vitro donde fueron analizados cortes en parafina de 12 incisivos de crecimiento continuo de ratones machos Swiss salvajes de 7 días de nacidos. Las etapas del desarrollo de la formación del esmalte dental se identificaron por medio de la coloración hematoxilina-eosina. La inmunodetención de los receptores de potencial transitorio de tipo vaniloide-5 fue efectuada por medio del anticuerpo primario policlonal anti-TRPV5. Las observaciones se llevaron a cabo mediante un microscopio Leica DM 500. Resultados. Se identificaron las diferentes etapas implicadas en la formación del esmalte dental, entre ellas, la etapa de secreción y maduración. En ellas, se evidenció que los ameloblastos eran células alargadas con núcleos ovalados en posición proximal y con áreas manifiestamente desarrolladas de secreción medial y distal. Fue visualizado un inmunomarcaje de TRPV5 en la membrana plasmática y citoplasma de los ameloblastos de secreción y maduración de todos los ratones analizados. Los TRPV5 también fueron  inmunolocalizado en los odontoblastos, endotelio vascular y células pulpares. Conclusiones. Los receptores de potencial transitorio de tipo vaniloide-5 se localizan en los ameloblastos de secreción y maduración de ratones Swiss. Particularmente, los TRPV5 son inmunodetectados en los odontoblastos, endotelio vascular y células de la pulpa dental.es-ES
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dc.languagespa
dc.languageeng
dc.publisherUniversidad de Córdobaes-ES
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/2681/4920
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/2681/4921
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/2681/4922
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/2681/4923
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/2681/4929
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dc.rightsDerechos de autor 2022 Victor Hugo Simancas-Escorcia, Antonio Díaz-Caballero, Adel Martinez-Martinezes-ES
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0es-ES
dc.sourceJournal MVZ Cordoba; Vol. 27 No. 3 (2022): Vol. 27 Núm. 3 (2022): Revista MVZ Córdoba Volumen 27(3) Septiembre-Diciembre 2022; e2681en-US
dc.sourceRevista MVZ Córdoba; Vol. 27 Núm. 3 (2022): Vol. 27 Núm. 3 (2022): Revista MVZ Córdoba Volumen 27(3) Septiembre-Diciembre 2022; e2681es-ES
dc.source1909-0544
dc.source0122-0268
dc.source10.21897/rmvz.v27.n3.2022
dc.subjectAmeloblastsen-US
dc.subjectTRPV Cation Channelsen-US
dc.subjectCalcium channelsen-US
dc.subjectOdontoblastsen-US
dc.subjectDental enamelen-US
dc.subjectDentinen-US
dc.subjectAmeloblastoses-ES
dc.subjectCanales Catiónicos TRPVes-ES
dc.subjectCanales de calcioes-ES
dc.subjectOdontoblastoses-ES
dc.subjectEsmalte dentales-ES
dc.subjectDentinaes-ES
dc.titleImmunohistochemical localization of transient receptor potential vanilloid-type 5 in ameloblasts of Swiss miceen-US
dc.titleLocalización inmunohistoquímica de receptores de potencial transitorio tipo vaniloide 5 en ameloblastos de ratones Swisses-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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