Infección reciente del virus del Oeste del Nilo en caballos del oriente de Yucatán, México

dc.creatorOrtíz-Esquivel, José E.
dc.creatorRosado-Aguilar, José Alberto
dc.creatorRodríguez-Vivas, Roger Iván
dc.creatorTorres-Castro, Marco
dc.creatorGutiérrez-Ruiz, Edwin José
dc.creatorBates-Acosta, Arturo
dc.creatorPuerto, Fernando I
dc.date2021-06-02
dc.date.accessioned2022-12-15T14:43:34Z
dc.date.available2022-12-15T14:43:34Z
dc.identifierhttps://revistamvz.unicordoba.edu.co/article/view/e2165
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5353452
dc.descriptionObjective. To determine the circulation of West Nile virus (WNV) in horses from the eastern Yucatán, Mexico. Materials and Methods. For convenience, 184 horses from 23 production units in the municipalities of Tizimín and Panabá, Yucatán, were studied. A blood serum sample was obtained from each studied horse and evaluated with an immunoglobulin M antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) to detect immunoglobulin M (IgM) against WNV. Additionally, positive reactor and suspect serum samples were analyzed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA. Results. Eight studied horses were seropositive (4.3%, 8/184) to WNV and two were suspects (1.1%, 2/184). All serum samples were negative by RT-PCR. Conclusions. The detection of IgM specific against WNV in the studied horses shows recent infections with the virus and indicates its circulation in eastern Yucatán, Mexico.en-US
dc.descriptionObjetivo. Determinar la circulación del virus del Oeste del Nilo (VON) en caballos del oriente de Yucatán, México. Materiales y métodos. Por conveniencia se estudiaron 184 caballos localizados en 23 unidades de producción de los municipios de Tizimín y Panabá, Yucatán. De cada caballo se obtuvo una muestra de suero sanguíneo, la cual fue evaluada con un ensayo de inmunoabsorción enzimática de captura (MAC-ELISA) para detectar inmunoglobulinas de clase M (IgM) específicas contra VON. Adicionalmente, los sueros seroreactores y los sospechosos fueron analizados con una reacción en cadena de la polimerasa por retrotranscripción (RT-PCR) para detectar ARN viral. Resultados. Ocho caballos estudiados fueron seroreactores en la ELISA-MAC (4.3%, 8/184) y dos fueron sospechosos (1.1%, 2/184). Todos estos sueros fueron negativos a la RT-PCR. Conclusiones. La detección de IgM específicas contra VON en los caballos estudiados, demuestra infecciones recientes e indica la circulación del virus en el oriente de Yucatán.es-ES
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dc.languagespa
dc.languageeng
dc.publisherUniversidad de Córdobaes-ES
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dc.rightsDerechos de autor 2021 José E. Ortíz-Esquivel, José Alberto Rosado-Aguilar, Roger Iván Rodríguez-Vivas, Marco Torres-Castro, Edwin José Gutiérrez-Ruiz, Arturo Bates-Acosta, Fernando I Puertoes-ES
dc.sourceJournal MVZ Cordoba; Vol. 26 No. 3 (2021): Journal MVZ Cordoba Volume 26(3) September-December 2021; e2165en-US
dc.sourceRevista MVZ Córdoba; Vol. 26 Núm. 3 (2021): Revista MVZ Córdoba Volumen 26(3) Septiembre-Diciembre 2021; e2165es-ES
dc.source1909-0544
dc.source0122-0268
dc.source10.21897/rmvz.v26.n3.2021
dc.subjectAntibodiesen-US
dc.subjectMAC-ELISAen-US
dc.subjectFLAVIVIRUSen-US
dc.subjectIgMen-US
dc.subjectSerologyen-US
dc.subjectAnticuerposes-ES
dc.subjectMAC-ELISAes-ES
dc.subjectFlaviviruses-ES
dc.subjectIgMes-ES
dc.subjectSerologíaes-ES
dc.titleRecent infection of West Nile Virus in horses from eastern Yucatán, Mexicoen-US
dc.titleInfección reciente del virus del Oeste del Nilo en caballos del oriente de Yucatán, Méxicoes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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