Selection markers in Pampa Rocha pigs: a comparison with autochthonous breeds of Spain and Portugal

Marcadores de selección en cerdos Pampa Rocha: comparación con razas autóctonas de España y Portugal

dc.creatorBurgos Serrano, Carmen
dc.creatorLlambí Dellacasa, Silvia
dc.creatorHidalgo Gracia, Jorge
dc.creatorMontenegro Silva, Maria
dc.creatorArruga Laviña, Victoria
dc.creatorLópez-Buesa, Pascual
dc.date2019-04-24
dc.date.accessioned2022-12-15T14:43:13Z
dc.date.available2022-12-15T14:43:13Z
dc.identifierhttps://revistamvz.unicordoba.edu.co/article/view/1642
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5353348
dc.descriptionObjective. The analysis of selection markers allows to obtain information about the evolutive story of a particular breed or line and allows also to evaluate the usefulness of those markers for breeding programs. We have analyzed SNPs in four genes of the creole pig breed Pampa Rocha and we have compared their allelic frequencies with the allelic frequencies of diverse autochthonous breeds of Spain and Portugal and also with Piétrain pigs and wild boars. Materials and methods. The SNPs were analyzed using diverse RT-PCR methods. Results. The results of the analysis show that Pampa Rocha pigs have similar allelic frequencies with the autochthonous breeds of Spain and Portugal especially in the case of IGF2 and also, but not so coincident, in the case of PEPCK-C. However, they differ considerably for MC4R, and also, but in a lower extent, for PRKAG3. We discuss in this work the usefulness of our results for breeding of Pampa Rocha pigs. Conclusions. Our results demonstrate the peculiarity of the Pampa Rocha breed regarding the markers studied.en-US
dc.descriptionObjetivo. El análisis de marcadores de selección permite obtener datos de la vida evolutiva de una raza o línea y permite también evaluar la conveniencia o no de su uso en programas de mejora genética. Hemos evaluado SNPs en cuatro genes (IGF2, MC4R, PRKAG3 y PEPCK-C), que tienen importantes efectos fenotípicos, en cerdos de la raza Pampa Rocha, una raza criolla, y hemos comparado sus frecuencias alélicas con cerdos de diversas razas autóctonas y líneas de España y Portugal no sometidas a selección así como con jabalíes y cerdos de la raza Piétrain. Materiales y métodos. Los SNPs fueron analizados mediante diversa técnicas de RT-PCR. Resultados. Los resultados de los análisis muestran una similitud de frecuencias alélicas entre los cerdos de la raza Pampa Rocha y los cerdos autóctonos de la península ibérica sobre todo en el gen IGF2 y, en menor medida en el gen PEPCK-C. Sin embargo difieren considerablemente en el caso del marcador MC4R y, también en menor medida, en PRKAG3. En el trabajo se discute el uso potencial de los resultados obtenidos para orientar la selección genética de cerdos de la raza Pampa Rocha. Conclusiones. Nuestros resultados demuestran la peculiaridad de la raza Pampa Rocha con respecto a los marcadores estudiados.es-ES
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.formatapplication/xml
dc.formatapplication/xml
dc.formatapplication/zip
dc.formatapplication/zip
dc.languagespa
dc.languageeng
dc.publisherUniversidad de Córdobaes-ES
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1642/1874
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1642/1875
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1642/2067
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1642/2068
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1642/2069
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1642/2070
dc.relation/*ref*/1. Latorre P, Burgos C, Hidalgo J, Varona L, Carrodeguas JA, López-Buesa, P. c.A2456C-substitution in Pck1 changes the enzyme kinetic and functional properties modifying fat distribution in pigs. Sci Rep. 2016; 6:19617. https://doi.org/10.1038/srep19617
dc.relation/*ref*/2. Font-i-Furnols M, Tous N, Esteve-Garcia E, Gispert M. Do all the consumers accept the marbling in the same way? The relation between visual and sensory acceptability of pork. Meat Sci. 2012; 91(4):448-453. https://doi.org/10.1016/j.meatsci.2012.02.030
dc.relation/*ref*/3. Reina R, López-Buesa P, Sánchez del Pulgar J, Ventanas J, García C. Effect of IGF-II (insulin-like growth factor-II) genotype on the quality of dry-cured hams and shoulders. Meat Sci. 2012; 92(4):562-568. https://doi.org/10.1016/j.meatsci.2012.05.027
dc.relation/*ref*/4. Pugliese C, Sirtori F. Quality of meat and meat products produced from southern European pig breeds. Meat Sci. 2012; 90(3):511-518. https://doi.org/10.1016/j.meatsci.2011.09.019
dc.relation/*ref*/5. Van Laere AS, Nguyen M, Braunschweig M, Nezer C, Collete C, Moreau L et al. A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature. 2003; 425(6960):832-836. https://doi.org/10.1038/nature02064
dc.relation/*ref*/6. Kim KS, Larsen N, Short T, Plastow G, Rothschild MF. A missense variant of the melanocortin-4-receptor (MC4R) gene is associated with fatness, growth, and feed intake traits. Mam Genome. 2000; 11(2):131–135. https://doi.org/10.1007/s003350010025
dc.relation/*ref*/7. Ciobanu D, Bastiaansen J, Massoud M, Helm J, Woollard J, Plastow G, Rothschild M. Evidence for new alleles in the protein kinase adenosine monophosphate-activated g3-subunit gene associated with low glycogen content in pig skeletal muscle and improved meat quality. Genetics. 2001; 159(3):1151–1162. PMID: 11729159
dc.relation/*ref*/8. Montenegro M, Llambí S, Castro G, Barlocco N, Vadell A, Landi V et al. Genetic characterization of Uruguayan Pampa Rocha pigs with microsatellite markers. Genet Mol Biol. 2015; 38(1):48-54. https://doi.org/10.1590/s1415-475738120140146
dc.relation/*ref*/9. Fuentes V, Ventanas S, Ventanas J, Estévez M. The genetic background affects composition, oxidative stability and quality traits of Iberian dry-cured hams: Purebred Iberian versus reciprocal Iberian × Duroc crossbred pigs. Meat Sci. 2014; 96(2):737-743. https://doi.org/10.1016/j.meatsci.2013.10.010
dc.relation/*ref*/10. Burgos C, Galve A, Moreno C, Altarriba J, Reina R, García C, López-Buesa. The effects of two alleles of IGF2 on fat content in pig carcasses and pork. Meat Sci. 2012; 90(2):309-313. https://doi.org/10.1016/j.meatsci.2011.07.016
dc.relation/*ref*/11. Burgos C, Carrodeguas JA, Moreno C, Altarriba J, Tarrafeta L, Barcelona JA et al. Allelic incidence in several pig breeds of a missense variant of pig melanocortin-4 receptor (MC4R) gene associated with carcass and productive traits; its relation to IGF2 genotype. Meat Sci. 2006; 73(1):144-150. https://doi.org/10.1016/j.meatsci.2005.11.007
dc.relation/*ref*/12. Galve A, Burgos C, Varona L, Carrodeguas JA, López-Buesa P. Allelic frequencies of PRKAG3 in several pig breeds and its technological consequences on a Duroc 9 Landrace-Large White cross. J Anim Breed Genet. 2013; 130(5):382-393. https://doi.org/10.1111/jbg.12042
dc.relation/*ref*/13. Saintilan R, Mérour I, Schwob S, Sellier P, Bidanel J, Gilbert H. Genetic parameters and halothane genotype effect for residual feed intake in Piétrain growing pigs. Livest Sci. 2011; 142(1-3):203-209. https://doi.org/10.1016/j.livsci.2011.07.013
dc.relation/*ref*/14. Van den Maagdenberg K, Stinckens A, Claeys E, Buys N, De Smet S. Effect of the insulin-like growth factor-II and RYR1 genotype in pigs on carcass and meat quality traits. Meat Sci. 2008; 80(2):293-303. https://doi.org/10.1016/j.meatsci.2007.12.008
dc.relation/*ref*/15. Li C, Bin Y, Curchoe C, Yang L, Feng D, Jiang Q et al. Genetic imprinting of H19 and IGF2 in domestic pigs (Sus scrofa). Anim Biotechnol. 2008; 19(1):22–27. https://doi.org/10.1080/10495390701758563
dc.relation/*ref*/16. Stinckens A, Mathur P, Janssens S, Bruggeman V, Onagbesan OM, Schroyen M et al. Indirect effect of IGF2 intron3 g.3072G>A mutation on prolificacy in sows. Anim Genet. 2010; 41(5):493-498. https://doi.org/10.1111/j.1365-2052.2010.02040.x
dc.relation/*ref*/17. Krashes MJ, Lowell BB, Garfield AS. Melanocortin-4 receptor-regulated energy homeostasis. Nat Neurosci. 2016; 19(2):206-219. https://doi.org/10.1038/nn.4202
dc.relation/*ref*/18. Shen WJ, Yao T, Kong X, Williams KW, Liu T. Melanocortin neurons: Multiple routes to regulation of metabolism. Biochim Biophys Acta. 2017; 1863(10 Pt A):2477-2485. https://doi.org/10.1016/j.bbadis.2017.05.007
dc.relation/*ref*/19. Davoli R, Braglia S, Valastro V, Annaratone C, Comella M, Zambonelli et al. Analysis of MC4R polymorphism in Italian Large White and Italian Duroc pigs: association with carcass traits. Meat Sci. 2012; 90(4):887-892. https://doi.org/10.1016/j.meatsci.2011.11.025
dc.relation/*ref*/20. Switonski M, Mankowska M, Salamon S. Family of melanocortin receptor (MCR) genes in mammals-mutations, polymorphisms and phenotypic effects. J Appl Genet. 2013; 54(4):461-472. https://doi.org/10.1007/s13353-013-0163-z
dc.relation/*ref*/21. Škrlep M, Kavar T, Santé-Lhoutellier V, Čandek-Potokar M. Effect of I199V polymorphism at PRKAG3 gene on carcass and meat quality traits in Slovenian commercial pigs. J Muscle Foods. 2009; 20(3):367-376. https://doi.org/10.1111/j.1745-4573.2009.00158.x
dc.relation/*ref*/22. Salas RC, Mingala C. Genetic Factors Affecting Pork Quality: Halothane and Rendement Napole Genes. Anim Biotechnol. 2017; 28(2):148-155. https://doi.org/10.1080/10495398.2016.1243550
dc.sourceJournal MVZ Cordoba; Vol. 24 No. 2 (2019): Revista MVZ Córdoba Volumen 24(2) Mayo-Agosto 2019; 7198-7202en-US
dc.sourceRevista MVZ Córdoba; Vol. 24 Núm. 2 (2019): Revista MVZ Córdoba Volumen 24(2) Mayo-Agosto 2019; 7198-7202es-ES
dc.source1909-0544
dc.source0122-0268
dc.subjectIGF2en-US
dc.subjectMC4Ren-US
dc.subjectPRKAG3en-US
dc.subjectPEPCKen-US
dc.subjectSus scrofaen-US
dc.subjectIGF2es-ES
dc.subjectMC4Res-ES
dc.subjectPRKAG3es-ES
dc.subjectPEPCKes-ES
dc.subjectSus scrofaes-ES
dc.titleSelection markers in Pampa Rocha pigs: a comparison with autochthonous breeds of Spain and Portugalen-US
dc.titleMarcadores de selección en cerdos Pampa Rocha: comparación con razas autóctonas de España y Portugales-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución