Detection of equine herpesvirus 1 and 4 and its association with latency-associated transcripts in naturally infected horses in Colombia

Detección de los herpesvirus equinos 1 y 4 y su relación con transcriptos asociados a la latencia en caballos infectados naturalmente en Colombia

dc.creatorVargas-Bermudez, Diana
dc.creatorCorredor F, Adriana
dc.creatorRamírez-Nieto, Gloria
dc.creatorVera A, Víctor
dc.creatorJaime C, Jairo
dc.date2018-09-01
dc.date.accessioned2022-12-15T14:43:02Z
dc.date.available2022-12-15T14:43:02Z
dc.identifierhttps://revistamvz.unicordoba.edu.co/article/view/1371
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5353297
dc.descriptionObjective. The objective of this study was to determine the presence of antibodies and viral genomes of EHV-1 and EHV-4, as well as to detect the presence of latency associated transcripts (LATs) in a selected population of Colombian horses. Materials and methods. Serum samples, submandibular lymph nodes and trigeminal ganglion were obtained from 50 horses and analyzed. Sera were evaluated for the presence of antibodies against EHV-1 and EHV-4 while tissues were initially evaluated for the presence of viral genome by nPCR. Finally, samples were used for the detection of LATs through RT-PCR. Results. In general, 6/50 samples showed antibodies to EHV-1 and 44/50 were positive for EHV-4. As for viral genome detection, 10/50 samples were positive for EHV-1 and 30/50 were positive for EHV-4; in addition, 22/35 horses positive for EHV DNA were positive for LATs. The use of these tests led to eight possible combinations of results. Conclusions. The evidence used shows that horses can have simple viral infection, co-infections with both viruses, latency due to the presence of LATs and the simultaneous presence of LATs and viral genome replication at a given time. It contributes to the understanding of the behavior of the disease in Colombia and calls attention to the importance of implementing complementary diagnoses to the serology for the control of these viruses.en-US
dc.descriptionObjetivo. El objetivo de este estudio fue determinar presencia de anticuerpos y genoma viral de EHV-1 y EHV-4, como también detectar la presencia de transcriptos asociados a latencia (LATs) en una población seleccionada de caballos colombianos. Materiales y métodos. Muestras de suero, nódulos linfáticos submandibulares y ganglio trigémino se obtuvieron de 50 caballos y fueron analizadas. Los sueros se evaluaron para la presencia de anticuerpos contra EHV-1 y EHV-4 mientras que los tejidos se evaluaron inicialmente para la presencia de genoma viral por nPCR. Finalmente, las muestras se emplearon para la detección de LATs a través de RT-PCR. Resultados. En general, 6/50 muestras mostraron anticuerpos para EHV-1 y 44/50 fueron positivos para EHV-4. En cuanto a la detección del genoma viral, 10/50 muestras fueron positivas para EHV-1 y 30/50 fueron positivas para EHV-4; además, 22/35 caballos positivos para DNA de EHV fueron positivos para LATs. El empleo de estas pruebas llevó a ocho posibles combinaciones de resultados. Conclusiones. Se confirma la presencia de estos virus en la población equina colombiana. Las pruebas empleadas demuestran que los caballos pueden tener infección viral simple, co-infecciones con ambos virus, estado de latencia debido a la presencia de los LATs y presencia simultánea de LATs y de replicación de genoma viral en un momento dado. Se aporta al entendimiento del comportamiento de la enfermedad en Colombia y se llama la atención sobre la importancia de implementar diagnósticos complementarios a la serología para el control de estos virus.es-ES
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dc.languagespa
dc.publisherUniversidad de Córdobaes-ES
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1371/pdf
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1371/epub
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1371/2506
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dc.sourceJournal MVZ Cordoba; Vol. 23 No. 3 (2018): Revista MVZ Córdoba Volumen 23(3) Septiembre-Diciembre 2018; 6826-6837en-US
dc.sourceRevista MVZ Córdoba; Vol. 23 Núm. 3 (2018): Revista MVZ Córdoba Volumen 23(3) Septiembre-Diciembre 2018; 6826-6837es-ES
dc.source1909-0544
dc.source0122-0268
dc.subjectAntibodiesen-US
dc.subjectEHV-1en-US
dc.subjectEHV-4en-US
dc.subjectLatencyen-US
dc.subjectPCRen-US
dc.subjectEHV-1es-ES
dc.subjectEHV-4es-ES
dc.subjectELISAes-ES
dc.subjectLatenciaes-ES
dc.subjectPCRes-ES
dc.titleDetection of equine herpesvirus 1 and 4 and its association with latency-associated transcripts in naturally infected horses in Colombiaen-US
dc.titleDetección de los herpesvirus equinos 1 y 4 y su relación con transcriptos asociados a la latencia en caballos infectados naturalmente en Colombiaes-ES
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dc.typeinfo:eu-repo/semantics/publishedVersion


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