Endothelin-1 expression in pulmonary hypertensive chickens by hypoxia

Expresión de Endotelina-1 en pollos con hipertensión arterial pulmonar por hipoxia hipobárica

dc.creatorMonroy G, Luis Carlos
dc.creatorHernández V, Aureliano
dc.date2017-05-02
dc.date.accessioned2022-12-15T14:42:38Z
dc.date.available2022-12-15T14:42:38Z
dc.identifierhttps://revistamvz.unicordoba.edu.co/article/view/1032
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5353184
dc.descriptionObjective. To determine the effect of hypobaric hypoxia exposition in pulmonary arterioles expression of endothelin-1 (ET-1). Materials and methods. Two groups of commercial broiler chickens were used: one of them were raised at 2638 (hypobaric hypoxia) and the other one at 300 m (relative normoxia) above sea level. Incidence of pulmonary hypertension (PH) was evaluated by calculating the cardiac mass index values and ET-1 protein expression was established in pulmonary arterioles by immunohistochemistry and morphometry. Results. ET-1 expression was higher in arterioles of animals exposed to hypoxia as compared to the low altitude exposed broilers (p<0.01). Arterioles from pulmonary hypertensive chickens (PHC) showed ET-1 higher expression than arterioles from healthy chickens (non-hypertensive, NHC) at low altitude, those exposed to hypobaric hypoxia (p<0.01). 53% of chickens subjected to altitude conditions developed pulmonary hypertension. Under normoxia, no chickens developed that pathology. Conclusions. Quantitative characteristics and sites of ET-1 expression in the lungs are important in the understanding of PH pathogenesis in broilers and the adapting mechanisms to hypobaric hypoxia, as to design new pharmacological approaches. This is a first approach which accounts for the abovementioned features in broilers subjected to natural conditions of normoxia and hypobaric hypoxia.en-US
dc.descriptionObjetivo. Determinar el efecto de la exposición a hipoxia hipobárica sobre la expresión de Endotelina-1 en arteriolash pulmonares. Material y métodos. Se utilizaron 2 grupos de pollos de engorde de una estirpe comercial: uno de ellos criados a 2638 y el otro, a 300 m de altitud. La incidencia de HAP se evaluó según los valores del índice de masa cardiaca y se compararon los niveles de expresión de la proteína ET-1 en arteriolas pulmonares de pollos de engorde sanos y enfermos por HAP mediante inmunohistoquímica y morfometría. Resultados. La expresión de la proteína ET-1 fue mayor en las arteriolas de los pollos expuestos a hipoxia hipobárica que en los criados bajo condiciones de normoxia relativa (p<0.01). Los animales enfermos por HAP presentaron mayor expresión de la proteína ET-1 en las arteriolas pulmonares que los animales sanos ubicados en las dos altitudes (p<0.01). 53% de los animales desarrollaron hipertensión pulmonar y ninguno de los mantenidos en normoxia lo hicieron. Conclusiones. El conocimiento de las características cuantitativas y lo sitios de expresión de la ET-1 son elementos importantes para entender aún más la patogenia de la HAP y el diseño de fármacos para su control. Este estudio constituye la primera aproximación cuantitativa relacionada con la expresión de ET-1 en pollos de engorde con HAP de origen hipóxico no inducida.es-ES
dc.formatapplication/pdf
dc.languagespa
dc.publisherUniversidad de Córdobaes-ES
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1032/pdf
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dc.sourceJournal MVZ Cordoba; Vol. 22 No. 2 (2017): Revista MVZ Córdoba Volumen 22(2) Mayo-Agosto 2017; 5951-5958en-US
dc.sourceRevista MVZ Córdoba; Vol. 22 Núm. 2 (2017): Revista MVZ Córdoba Volumen 22(2) Mayo-Agosto 2017; 5951-5958es-ES
dc.source1909-0544
dc.source0122-0268
dc.subjectArteriolesen-US
dc.subjectendothelin-1en-US
dc.subjectpulmonary hypertensionen-US
dc.subjectmorphometryen-US
dc.subjectimmunohistochemistryen-US
dc.subjectArteriolases-ES
dc.subjectendotelina-1es-ES
dc.subjecthipertensión pulmonares-ES
dc.subjectmorphometríaes-ES
dc.subjectinmunohistoquímicaes-ES
dc.titleEndothelin-1 expression in pulmonary hypertensive chickens by hypoxiaen-US
dc.titleExpresión de Endotelina-1 en pollos con hipertensión arterial pulmonar por hipoxia hipobáricaes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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