Evaluation of Lactobacillus plantarum in large intestine of piglets by transmission microscopy and blood chemistry

Evaluación de Lactobacillus plantarum en intestino grueso de lechones por microscopía electrónica y química sanguínea

dc.creatorJurado G, Henry
dc.creatorCastaño Z, Diana
dc.creatorRamírez T, Cristina
dc.date2011-05-10
dc.date.accessioned2022-12-15T14:42:34Z
dc.date.available2022-12-15T14:42:34Z
dc.identifierhttps://revistamvz.unicordoba.edu.co/article/view/1018
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5353170
dc.descriptionObjective. To evaluate the presence of Lactobacillus plantarum in large intestine of piglets. Materials and methods. Fifty piglets were randomly allocated into 5 (n=10) treatment groups (T0: without probiotics; T1:with L. plantarum 1 H1; T2:with L. plantarum 1 H2; T3: with commercial probiotics; T4:without commercial probiotics). After treatment, 3 piglets, from each group, were randomly selected and large intestine samples were taken and analyzed by transmission electron microscopy and serum for blood chemistry. The determination of immunoglobulin A (IgA) was made by turbidimetric method; and the total cholesterol and blood ureic nitrogen (BUN) by espectrophotometry. The differential leukocyte count and polymorphonuclear neutrophils (PMN) was examined in blood smears. Results. Adhesion of L. plantarum 1 H1 and L. plantarum 1 H2 was found in the mucosa of the large piglet intestine. Was observed secretion of mucin, and inflammation in the lamina propria and connective tissue edema as part of the immune response. IgA showed high concentrations in T2 (L. plantarum 1 H1, 333 mg/100 ML) and T3 (L. plantarum 1 H2, 300 mg/100 mL). The T2 and T3 values in polymorphonuclear neutrophils were elevated (65% and 55% respectively). Total cholesterol was lower in T2 (113.83%) and T3 (93.8%). T2 and T3 showed the lowest BUN values (7.83 and 8.76 mg% respectively). Conclusions. The use of probiotics with L. plantarum 1 added in the ration showed a positive effect in the colonization and adhesion in the large intestine, as well as, a positive response in their immune system.en-US
dc.descriptionObjetivo. Evaluar la presencia de Lactobacillus plantarum en intestino grueso de lechones. Materiales y métodos. 50 lechones fueron asignados al azar a 5 tratamientos (n=10). (T0: sin probiótico; T1: con L. plantarum 1 H1; T2: con L. plantarum 1 H2; T3: con probiótico comercial; T4: sin probiótico comercial). Después de los tratamientos, 3 lechones de cada grupo fueron seleccionados aleatoriamente para obtener muestras de intestino grueso para análisis por microscopía electrónica y suero para química sanguínea. La inmunoglobulina A (IgA) se hizo por turbidimetría; colesterol total y nitrógeno ureico (BUN) por espectofotometría. El recuento diferencial de leucocitos y polimorfonucleares (PMN) neutrófilos mediante extendido de sangre. Resultados. Se comprobó la adhesión de L. plantarum 1 H1 y y L. plantarum 1 H2 en el intestino grueso. Se observó secreción de mucina y en la lámina propia inflamación y edema del tejido conectivo. La IgA mostró concentraciones altas en T2 (L. plantarum 1 H1) con 333 mg/100 mL y T3 (L. plantarum 1 H2) con 300 mg/100 mL. Los valores en T3 y T2 en los polimorfonucleares neutrófilos fueron elevados (65% y 55% respectivamente). El colesterol total fue menor en T2 y T3 con valores de 113.83 y 93.8 mg% respectivamente. El BUN para T2 y T3 fue el más bajo con 7.83 y 8.76 mg% respectivamente. Conclusiones. La utilización de probióticos con L. plantarum 1 adicionado en la ración mostró un efecto positivo en la colonización y adhesión en el intestino grueso, así como, una respuesta positiva en su sistema inmune.es-ES
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dc.languagespa
dc.publisherUniversidad de Córdobaes-ES
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/1018/1244
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dc.sourceJournal MVZ Cordoba; Vol. 16 No. 2 (2011): Revista MVZ Córdoba Volumen 16(2) Mayo-Agosto 2011; 2538-2548en-US
dc.sourceRevista MVZ Córdoba; Vol. 16 Núm. 2 (2011): Revista MVZ Córdoba Volumen 16(2) Mayo-Agosto 2011; 2538-2548es-ES
dc.source1909-0544
dc.source0122-0268
dc.subjectInmunoglobulinases-ES
dc.subjectLactobacillus plantarumes-ES
dc.subjectlechónes-ES
dc.subjectprobióticoses-ES
dc.subjectsistema inmunológicoes-ES
dc.titleEvaluation of Lactobacillus plantarum in large intestine of piglets by transmission microscopy and blood chemistryen-US
dc.titleEvaluación de Lactobacillus plantarum en intestino grueso de lechones por microscopía electrónica y química sanguíneaes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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