Phenotypic characterization of canine Malassezia spp., isolates

Caracterización fenotípica de aislamientos de Malassezia spp., de origen canino

dc.creatorHurtado-Suárez, Angélica
dc.creatorPulido-Villamarín, Adriana
dc.creatorLinares-Linares, Melva
dc.creatorSuárez-Fernández, Leidy
dc.creatorCastañeda-Salazar, Rubiela
dc.creatorRodríguez-Bocanegra, María
dc.date2016-09-01
dc.date.accessioned2022-12-15T14:42:26Z
dc.date.available2022-12-15T14:42:26Z
dc.identifierhttps://revistamvz.unicordoba.edu.co/article/view/827
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5353127
dc.descriptionABSTRACTObjective. To characterize and identify yeasts of the genus Malassezia by phenotypic features. Materials and methods. First, the macroscopic and microscopic morphological characteristics were described. In addition we performed biochemical and physiological assays as Tweens and Cremophor, including more. Results. Our results evidenced of 105 isolates obtained from dogs diagnosed with external otitis, it was possible to identify two distinct species from 46 isolates within the Malassezia genus: 36.19% (n=38) were identified as M. pachydermatis and 7.62% (n=8) as M. furfur. According to phenotypic patterns the remaining 56.19% (n=59) were reported as Malassezia spp., possibly corresponding to M. furfur and/or M. pachydermatis. Conclusions. Results emphasize the necessity to characterize according to species. It is not feasible to define Malassezia by species based on morphological, biochemical, and physiological findings. Therefore, molecular genotyping should be performed to identify markers allowing a more precise isolate identification. This would broaden our epidemiological knowledge regarding different species involved in canine otitis pathologiesen-US
dc.descriptionRESUMENObjetivo. Caracterizar e identificar levaduras del género Malassezia, mediante características fenotípicas. Materiales y métodos. Inicialmente se describieron las características morfológicas macroscópicas y microscópicas, adicionalmente se realizaron pruebas bioquímicas y fisiológicas como Tween y Cremophor, entre otras. Resultados. De 105 aislamientos de caninos diagnosticados previamente con otitis, 46 fueron caracterizados hasta especie, así: El 36.19% (n=38) correspondió a M. pachydermatis, el 7.2% (n=8) a M. furfur; y 56.19% (n=59) restante fueron reportados como Malassezia spp., debido a los patrones fenotípicos atípicos que presentaron, y que podrían corresponder a variantes de M. furfur y/o M. pachydermatis. Conclusión. Estos resultados enfatizan la necesidad de hacer una caracterización a nivel de especie y/o genotipos mediante marcadores moleculares que permitan una identificación más precisa de los aislamientos. Con el presente estudio, se contribuye al conocimiento de las diferentes especies involucradas en patologías óticas en caninos.es-ES
dc.formatapplication/pdf
dc.languagespa
dc.publisherUniversidad de Córdobaes-ES
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/827/937
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dc.sourceJournal MVZ Cordoba; Vol. 21 No. 3 (2016): Revista MVZ Córdoba Volumen 21(3) Septiembre-Diciembre 2016; 5535-5546en-US
dc.sourceRevista MVZ Córdoba; Vol. 21 Núm. 3 (2016): Revista MVZ Córdoba Volumen 21(3) Septiembre-Diciembre 2016; 5535-5546es-ES
dc.source1909-0544
dc.source0122-0268
dc.subjectBiochemical techniquesen-US
dc.subjectexternal otitisen-US
dc.subjectMalasseziaen-US
dc.subjectmorphologyen-US
dc.subjectMalasseziaes-ES
dc.subjectmorfologíaes-ES
dc.subjectotitis externaes-ES
dc.subjectpruebas bioquímicases-ES
dc.titlePhenotypic characterization of canine Malassezia spp., isolatesen-US
dc.titleCaracterización fenotípica de aislamientos de Malassezia spp., de origen caninoes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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