| dc.description.abstract | The milk represents one of dietary sources of nutrients for humans due to its nutritional composition rich in high biological value proteins, lipids, minerals and vitamins. However, consumption is related to the ingestion of quimical contaminants such as mycotoxins. The aflatoxin B1 (AFB1) is the most prevalent and the most toxic and when ingested by domestic animals, as dairy cattle, through contaminated feed intake, is excreted in milk, tissues and biological fluids of these animals. Considering these contaminants are stable to heat treatments, the use of micro-organisms through its binding capacity and / or inhibition with aflatoxins, can promote the reduction of the available levels for absorption and distribution in the body have attracted the attention of researchers. The highlight is the lactic acid bacteria (LAB) widely used in the dairy industry can also provide greater nutrient availability and possible reduction of contaminants. Regarding prebiotics, few studies have evaluated the decontamination of action of these compounds and it is suggested further that the probiotics to be synergistic in the removal of mycotoxins. In this context, the study aimed to evaluate the effect of the probiotic micro-organism of Lactobacillus and the influence of several prebiotics inulin, beta-glucan, polydextrose and oligofructose, alone or in combination, in milk artificially contaminated with AFB1, to evaluate the reduction of that mycotoxin and bioavailability after digestibility in a in vitro digestion model. Lactobacillus plantarum, was inoculated in spiked samples with AFB1 (10 μg.L-1) with prebiotic compounds, isolated and combined, a total of 12 different assays. The bioaccessibility was determined using a simulated gastrointestinal digestion that mimics the physiological conditions of the digestive tract until the colonic compartment. The tests showed capacity to remove the AFB1 from the environment, ranging from 7,56% to 55,85%. The test carried out in the presence of L.plantarum, inulin, oligofructose and betaglucan showed the reduction (56%) in spiked milk. The bioaccessibility of Afs from milk matrix ranged from 15,62 to 35,63% for AFB1. The best result for the bioaccessibility was demonstrated when introduced prebiotic inulin or oligofructose individually. | |
