dc.contributor | Rosa, Mauricio Ferreira da | |
dc.contributor | Favreto, Wagner Alex Jann | |
dc.contributor | Rosa, Mauricio Ferreira da | |
dc.contributor | Favreto, Wagner Alex Jann | |
dc.contributor | Maniglia, Thiago Cintra | |
dc.contributor | Coutinho, Lincoln Figueira Marins | |
dc.creator | Pinto, Sandra Cristina Girotto | |
dc.date.accessioned | 2021-01-04T19:15:41Z | |
dc.date.accessioned | 2022-12-06T15:16:55Z | |
dc.date.available | 2021-01-04T19:15:41Z | |
dc.date.available | 2022-12-06T15:16:55Z | |
dc.date.created | 2021-01-04T19:15:41Z | |
dc.date.issued | 2019-06-07 | |
dc.identifier | PINTO, Sandra Cristina Girotto. Determinação de aciclovir em plasma humano através de precipitação de proteínas por UHPLC-ESI-MS/MS: desenvolvimento, validação e aplicação em estudo de bioequivalência. 2019. Trabalho de Conclusão de Curso (Especialização em Análise Instrumental) – Universidade Tecnológica Federal do Paraná, Toledo, 2019. | |
dc.identifier | http://repositorio.utfpr.edu.br/jspui/handle/1/23668 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5263490 | |
dc.description.abstract | A sensitive ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) method for measurements of acyclovir in plasma was developed and validated. A protein precipitation extraction was proposed for the clean up of plasma and entecavir was added as internal standard (IS). The analyses were carried out using a C18 column and mobile phase of 0.1% formic acid:methanol + 0.1% formic acid in gradient mode of proportion. The mass spectrometer equipped with an electrospray ion source in positive mode, was set up in selective reaction monitoring, to detect precursor → product ion with 226 > 152 m/z and 278 > 152 m/z transitions, for analyte and IS, respectively. The linearity was checked between 4 to 1000 ng/mL for acyclovir, with R2=0.998333. Satisfactory selectivity, linearity, precision, accuracy and showed no matrix effect or carryover were obtained for the UPLC-ESI-MS/MS method. The proposed method was successfully applied to a bioequivalence study of healthy human volunteers. | |
dc.publisher | Universidade Tecnológica Federal do Paraná | |
dc.publisher | Toledo | |
dc.publisher | Brasil | |
dc.publisher | Análise Instrumental | |
dc.publisher | UTFPR | |
dc.rights | openAccess | |
dc.subject | Espectrometria de massa | |
dc.subject | Cromatografia a líquido de alta eficiência | |
dc.subject | Medicamentos - Pesquisa | |
dc.subject | Mass spectrometry | |
dc.subject | High performance liquid chromatography | |
dc.subject | Drugs - Research | |
dc.title | Determinação de aciclovir em plasma humano através de precipitação de proteínas por UHPLC-ESI-MS/MS: desenvolvimento, validação e aplicação em estudo de bioequivalência | |
dc.type | specializationThesis | |