| dc.contributor | Baron, Alessandra Machado | |
| dc.contributor | Garcia, Patrícia Salomão | |
| dc.contributor | Andrade, Milena Martins | |
| dc.contributor | Baron, Alessandra Machado | |
| dc.contributor | Bonafe, Elton Guntendorfer | |
| dc.contributor | Samulewski, Rafael Block | |
| dc.creator | Castro, Michael Conceição de | |
| dc.date.accessioned | 2022-12-09 | |
| dc.date.accessioned | 2021-07-13T16:12:14Z | |
| dc.date.accessioned | 2022-12-06T14:09:23Z | |
| dc.date.available | 2022-12-09 | |
| dc.date.available | 2021-07-13T16:12:14Z | |
| dc.date.available | 2022-12-06T14:09:23Z | |
| dc.date.created | 2022-12-09 | |
| dc.date.created | 2021-07-13T16:12:14Z | |
| dc.date.issued | 2020-09-11 | |
| dc.identifier | CASTRO, Michael Conceição de. Produção de biofilmes para imobilização de lipases. 2020. Trabalho de Conclusão de Curso (Licenciatura em Química) – Universidade Tecnológica Federal do Paraná, Apucarana, 2020. | |
| dc.identifier | http://repositorio.utfpr.edu.br/jspui/handle/1/25567 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5242266 | |
| dc.description.abstract | In this study, an eco-friendly film (cassava starch, polyvinyl alcohol and sericin blend-AM-PVA-SS) was synthesized, characterized and applied as a novel support for Botryosphaeria ribisEC-01 lipase immobilization by adsorption. Measured film thicknesses were between 230 and 309 μm and exhibited increased flexibility and malleability over film without sericin. Based on p-nitrophenyl palmitate (pNPP) hydrolysis reaction, the activity retention of immobilized lipase was 987%. For optimal conditions, the yield in ethyl oleate was 95% for immobilized enzyme and 21 ± 5 (24 h) 68 ± 12% (48 h) for free enzyme. Maximum yield was obtained at 49 °C, molar ratio oleic acid:ethanol of 1:3, 1.25 g lipase-film or 50 U and 30 h. Even after 7 cycles of use immobilized lipase showed 52% reduction in ester yield. Eco-friendly film is a promising material as support to immobilize lipases and application in biocatalysis. | |
| dc.publisher | Universidade Tecnológica Federal do Paraná | |
| dc.publisher | Apucarana | |
| dc.publisher | Brasil | |
| dc.publisher | Licenciatura em Química | |
| dc.publisher | UTFPR | |
| dc.rights | embargoedAccess | |
| dc.subject | Enzimas imobilizadas | |
| dc.subject | Enzimas - Aplicações industriais | |
| dc.subject | Catalisadores | |
| dc.subject | Immobilized enzymes | |
| dc.subject | Enzymes - Industrial applications | |
| dc.subject | Catalysts | |
| dc.title | Produção de biofilmes para imobilização de lipases | |
| dc.type | bachelorThesis | |
