| dc.creator | Piñeiro Avila, Gerardo | |
| dc.creator | Salvador, Amparo | |
| dc.creator | De la Guardia, Miguel | |
| dc.date | 2011-04-06 | |
| dc.date.accessioned | 2022-11-04T22:57:21Z | |
| dc.date.available | 2022-11-04T22:57:21Z | |
| dc.identifier | https://produccioncientificaluz.org/index.php/ciencia/article/view/9033 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5129266 | |
| dc.description | An alternative method has been developed for the spectrophotometric determination of coprostanol in sediments, based on its extraction with chloroform-methanol(2+1 v/v) and enzymatic analysis in pH 7.0 buffer saturated toluene, using a bienzymic reactor prepared with 1 mg cholesterol oxidase (23 IU) and 1 mg horseradish peroxidase (220 PU) both noncovalently immobilised on controlled pore glass beads. The method allow us to determine coprostanol in a dynamic range from 2.2xl0-4 M to 9x10-4 M , measuring the absorbance at 458 nm of the oxidized form of p-anisidine. The relationship between absorbance (A) and coprostanol concentration (C) expressed in mol.L-1 was A = (0.0059 Ó 0.0005) + (17 Ó 1) (C) with a r2 = 0.998 and it was obtained a limit of detection of 7x10-6 M in the best experimental conditions. Recovery studies carried out on water and beach sediments, at concentration levels from 0.1 to 0.25 mg.g1, provided values from 96 to 112%. | es-ES |
| dc.format | application/pdf | |
| dc.language | spa | |
| dc.publisher | Facultad Experimental de Ciencias de la Universidad del Zulia | es-ES |
| dc.relation | https://produccioncientificaluz.org/index.php/ciencia/article/view/9033/9023 | |
| dc.rights | Derechos de autor 2016 Ciencia | es-ES |
| dc.source | Ciencia; Vol. 8 Núm. 1 | es-ES |
| dc.source | 1315-2076 | |
| dc.subject | coprostanol determination | es-ES |
| dc.subject | enzyrnatic analysis in non-aqueous media | es-ES |
| dc.subject | FiA | es-ES |
| dc.subject | sediments | es-ES |
| dc.title | Enzymatic flow injection analysis of coprostanol in non-aqueous media | es-ES |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion | |
