dc.contributorSmircich, Pablo. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología
dc.contributorGarat, Beatríz. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología
dc.creatorSmircich, Pablo
dc.creatorEl-Sayed, N.M
dc.creatorGarat, Beatriz
dc.date.accessioned2019-10-02T22:14:49Z
dc.date.accessioned2022-10-28T19:55:49Z
dc.date.available2019-10-02T22:14:49Z
dc.date.available2022-10-28T19:55:49Z
dc.date.created2019-10-02T22:14:49Z
dc.date.issued2017
dc.identifierSmircich, P., El-Sayed, N.M., Garat, B.Intrinsic DNA curvature in trypanosomes. BMC Research Notes, 2017, 10 (1), art. no. 585. doi: 10.1186/s13104-017-2908-y
dc.identifier1756-0500
dc.identifierhttps://hdl.handle.net/20.500.12008/22087
dc.identifier10.1186/s13104-017-2908-y
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4976264
dc.description.abstractBackground: Trypanosoma cruzi and Trypanosoma brucei are protozoan parasites causing Chagas disease and African sleeping sickness, displaying unique features of cellular and molecular biology. Remarkably, no canonical signals for RNA polymerase II promoters, which drive protein coding genes transcription, have been identified so far. The secondary structure of DNA has long been recognized as a signal in biological processes and more recently, its involvement in transcription initiation in Leishmania was proposed. In order to study whether this feature is conserved in trypanosomatids, we undertook a genome wide search for intrinsic DNA curvature in T. cruzi and T. brucei. Results: Using a region integrated intrinsic curvature (RIIC) scoring that we previously developed, a non-random distribution of sequence-dependent curvature was observed. High RIIC scores were found to be significantly correlated with transcription start sites in T. cruzi, which have been mapped in divergent switch regions, whereas in T. brucei, the high RIIC scores correlated with sites that have been involved not only in RNA polymerase II initiation but also in termination. In addition, we observed regions with high RIIC score presenting in-phase tracts of Adenines, in the subtelomeric regions of the T. brucei chromosomes that harbor the variable surface glycoproteins genes. Conclusions: In both T. cruzi and T. brucei genomes, a link between DNA conformational signals and gene expression was found. High sequence dependent curvature is associated with transcriptional regulation regions. High intrinsic curvature also occurs at the T. brucei chromosome subtelomeric regions where the recombination processes involved in the evasion of the immune host system take place. These findings underscore the relevance of indirect DNA readout in these ancient eukaryotes.
dc.languageen
dc.publisherBioMed Central Ltd.
dc.relationBMC Research Notes, 2017, 10 (1), art. no. 585
dc.rightsLicencia Creative Commons Atribución (CC –BY 4.0)
dc.rightsLas obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad De La República. (Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)
dc.subjectBase J
dc.subjectBrucei
dc.subjectCruzi
dc.subjectDNA intrinsic curvature
dc.subjectTranscription
dc.subjectTrypanosoma
dc.subjectVSG
dc.titleIntrinsic DNA curvature in trypanosomes
dc.typeArtículo


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